The prominin-like Gene Expressed in a Subset of Dopaminergic Neurons Regulates Locomotion in Drosophila
CD133, also known as prominin-1, was first identified as a biomarker of mammalian cancer and neural stem cells. Previous studies have shown that the ( ) gene, an orthologue of mammalian CD133 in , plays a role in glucose and lipid metabolism, body growth, and longevity. Because locomotion is require...
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Published in | Molecules and cells Vol. 45; no. 9; pp. 640 - 648 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Korean Society for Molecular and Cellular Biology
30.09.2022
한국분자세포생물학회 |
Subjects | |
Online Access | Get full text |
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Summary: | CD133, also known as prominin-1, was first identified as a biomarker of mammalian cancer and neural stem cells. Previous studies have shown that the
(
) gene, an orthologue of mammalian CD133 in
, plays a role in glucose and lipid metabolism, body growth, and longevity. Because locomotion is required for food sourcing and ultimately the regulation of metabolism, we examined the function of
in
locomotion. Both
mutants and pan-neuronal
inhibition flies displayed reduced spontaneous locomotor activity. As dopamine is known to modulate locomotion, we also examined the effects of
inhibition on the dopamine concentration and mRNA expression levels of tyrosine hydroxylase (TH) and DOPA decarboxylase (Ddc), the enzymes responsible for dopamine biosynthesis, in the heads of flies. Compared with those in control flies, the levels of dopamine and the mRNAs encoding TH and Ddc were lower in
mutant and pan-neuronal
inhibition flies. In addition, an immunostaining analysis revealed that, compared with control flies,
mutant and pan-neuronal
inhibition flies had lower levels of the TH protein in protocerebral anterior medial (PAM) neurons, a subset of dopaminergic neurons. Inhibition of
in these PAM neurons reduced the locomotor activity of the flies. Overall, these findings indicate that
expressed in PAM dopaminergic neurons regulates locomotion by controlling dopamine synthesis in
. |
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Bibliography: | These authors contributed equally to this work. https://doi.org/10.14348/molcells.2022.0006 |
ISSN: | 1016-8478 0219-1032 |
DOI: | 10.14348/molcells.2022.0006 |