Effect and Mechanism of Bushen Huoxue Recipe on Ovarian Reserve in Mice with Premature Ovarian Failure

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 36; no. 4; pp. 571 - 575
• Main Author: 宋坤琨 马雯雯 黄聪 丁嘉慧 崔丹丹 谈秀娟 肖静 张明敏
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.08.2016; Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
• Subjects: Animals; Bone Marrow - drug effects; Bone Marrow - metabolism; Cyclophosphamide - toxicity; Disease Models, Animal; Drugs, Chinese Herbal - administration & dosage; Estrous Cycle - drug effects; Female; Gene Expression Regulation - drug effects; Humans; Medicine; Medicine & Public Health; Mice; Ovarian Follicle - drug effects; Ovarian Follicle - growth & development; Ovarian Reserve - drug effects; Primary Ovarian Insufficiency - chemically induced; Primary Ovarian Insufficiency - drug therapy; Primary Ovarian Insufficiency - pathology; 储备; 卵巢功能; 小鼠; 早衰; 机制; 治疗效果; 蛋白水平; 补肾活血; Bushen Huoxue; Mouse Vasa Homologue; ovarian reserve; premature ovarian failure; neo-oogenesis
• ISSN: 1672-0733; 1993-1352; 1993-1352
• DOI: 10.1007/s11596-016-1627-2

The aim of the present study was to explore the effect and mechanism of Bushen Huoxue recipe（BHR） on ovarian reserve in mice with premature ovarian failure（POF）. Mice were divided into 3 groups： normal group, model group and BHR group. Intraperitoneal injection of cyclophosphamide was performed to create the POF model. Primordial follicular（PDF） number, ovarian wet weight, ovarian index, and estrous cycle were analyzed to evaluate the effect of BHR on POF. Meanwhile, the m RNA and protein level of Mouse Vasa Homologue（MVH） in the bone marrow, peripheral blood and ovary were detected, to explore the underlying mechanism of the treatment efficacy of BHR on ovarian reserve. By the time of BHR treatment for 28 days, BHR increased the PDF number and shortened the estrous cycle of POF mice. BHR also decreased the m RNA level of MVH in the bone marrow, and increased m RNA and protein level of MVH in the ovary of POF mice. Our results demonstrated a treatment efficacy of BHR on POF mice, and revealed that BHR might repair the dysfunction of germline stem cells in the bone marrow, and thus to improve the ovarian reserve and enhance the ovarian function of POF mice through neo-oogenesis.