Pharmacodynamic screening and simulation study of anti-hypoxia active fraction of Xiangdan injection

• Published in: Journal of ethnopharmacology Vol. 127; no. 1; pp. 103 - 107
• Main Authors: Shen, Zhi-Bin, Yin, Yong-Qin, Tang, Chun-Ping, Yan, Chao-Yan, Chen, Chao, Guo, Li-Bing
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.01.2010; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Active fraction; animal models; Animals; Biological and medical sciences; Chemical Fractionation; Chromatography, High Pressure Liquid; Computer Simulation; Drug Evaluation, Preclinical; drug formulations; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - pharmacology; extracts; Female; fractionation; General pharmacology; herbal medicines; hypoxia; Hypoxia - drug therapy; Hypoxia - mortality; injection; Injections, Intraperitoneal; Male; Medical sciences; medicinal plants; mice; Oriental traditional medicine; Pharmacodynamic action; Pharmacognosy. Homeopathy. Health food; pharmacokinetics; Pharmacology. Drug treatments; Protective Agents - chemistry; Protective Agents - pharmacology; Random Allocation; Rats; Simulation; simulation models; Solvents; Time Factors; Xiangdan injection; Xiangdan injection; Active fraction; Simulation; Pharmacodynamic action; Screening; Oxygen; Pharmacognosy; Plant origin; Hypoxia; Biological activity; Medicinal plant
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2009.09.041

The herbal formula Xiangdan injection and its modifications have been used in traditional Chinese medicine for about hundreds years to alleviate pain and promoting blood. To investigate the anti-hypoxia active fraction of Xiangdan injection. Xiangdan injection was extracted by ligarine, chloroform, acetic ether, n-butanol and water, represented respectively by A, B, C, D and E. Five extractions were group on the L12(2 5) orthogonal designed table. NIH mices anti-hypoxia experiment under normal pressure with rats was adopted on the basis of orthogonal design, prediction pharmacodynamics model of TCM prescriptions was established, and the simulation bias was evaluated by combining two scatterplots. The anti-hypoxia active fraction of Xiangdan injection was determined by experiments. The prediction model of TCM prescriptions established in this study can predict the drug actions for different formulas, and PE% ≤ 25% was observed in 112 mice (93.3%). As indicated in model prediction and experimental confirmation, the pharmacodynamic actions of the 5 formulas are all superior to that of the original formula, and ADE has the best effect.