Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction

• Published in: The Journal of immunology (1950) Vol. 189; no. 11; pp. 5336 - 5346
• Main Authors: Cunnington, Aubrey J, Njie, Madi, Correa, Simon, Takem, Ebako N, Riley, Eleanor M, Walther, Michael
• Format: Journal Article
• Language: English
• Published: United States 01.12.2012
• Subjects: Acute Disease; Antigens, CD - blood; Antigens, Differentiation, Myelomonocytic - blood; Child; Child, Preschool; Coinfection; Erythrocyte Count; Female; Gene Expression; Haptoglobins - analysis; Heme - analysis; Heme Oxygenase-1 - genetics; Heme Oxygenase-1 - immunology; Hemolysis - immunology; Hemopexin - analysis; Humans; Malaria, Falciparum - immunology; Malaria, Falciparum - parasitology; Malaria, Falciparum - pathology; Male; Neutrophils - immunology; Neutrophils - metabolism; Neutrophils - pathology; Plasmodium falciparum; Plasmodium falciparum - immunology; Receptors, Cell Surface - blood; Respiratory Burst - immunology; Salmonella; Salmonella - immunology; Salmonella Infections - immunology; Salmonella Infections - microbiology; Salmonella Infections - pathology; Up-Regulation
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.1201028

It is not known why people are more susceptible to bacterial infections such as nontyphoid Salmonella during and after a malaria infection, but in mice, malarial hemolysis impairs resistance to nontyphoid Salmonella by impairing the neutrophil oxidative burst. This acquired neutrophil dysfunction is a consequence of induction of the cytoprotective, heme-degrading enzyme heme oxygenase-1 (HO-1) in neutrophil progenitors in bone marrow. In this study, we assessed whether neutrophil dysfunction occurs in humans with malaria and how this relates to hemolysis. We evaluated neutrophil function in 58 Gambian children with Plasmodium falciparum malaria [55 (95%) with uncomplicated disease] and examined associations with erythrocyte count, haptoglobin, hemopexin, plasma heme, expression of receptors for heme uptake, and HO-1 induction. Malaria caused the appearance of a dominant population of neutrophils with reduced oxidative burst activity, which gradually normalized over 8 wk of follow-up. The degree of neutrophil impairment correlated significantly with markers of hemolysis and HO-1 induction. HO-1 expression was increased in blood during acute malaria, but at a cellular level HO-1 expression was modulated by changes in surface expression of the haptoglobin receptor (CD163). These findings demonstrate that neutrophil dysfunction occurs in P. falciparum malaria and support the relevance of the mechanistic studies in mice. Furthermore, they suggest the presence of a regulatory pathway to limit HO-1 induction by hemolysis in the context of infection and indicate new targets for therapeutic intervention to abrogate the susceptibility to bacterial infection in the context of hemolysis in humans.