Ketogenic diet poses a significant effect on imbalanced gut microbiota in infants with refractory epilepsy

AIM To investigate whether patients with refractory epilepsy and healthy infants differ in gut microbiota(GM),and how ketogenic diet(KD) alters GM.METHODS A total of 14 epileptic and 30 healthy infants were recruited and seizure frequencies were recorded. Stool samples were collected for 16 S r DNA...

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Published inWorld journal of gastroenterology : WJG Vol. 23; no. 33; pp. 6164 - 6171
Main Authors Xie, Gan, Zhou, Qian, Qiu, Chuang-Zhao, Dai, Wen-Kui, Wang, He-Ping, Li, Yin-Hu, Liao, Jian-Xiang, Lu, Xin-Guo, Lin, Su-Fang, Ye, Jing-Hua, Ma, Zhuo-Ya, Wang, Wen-Jian
Format Journal Article
LanguageEnglish
Published United States Baishideng Publishing Group Inc 07.09.2017
Subjects
Bacteroides - isolation & purification
Child, Preschool
Cronobacter - isolation & purification
Diet, Ketogenic - adverse effects
Drug Resistant Epilepsy - diet therapy
Drug Resistant Epilepsy - microbiology
Female
Follow-Up Studies
Gastrointestinal Microbiome - physiology
Humans
Infant
Infant, Newborn
Intestines - microbiology
Male
Proteobacteria - isolation & purification
Retrospective Study
Seizures - diet therapy
Seizures - microbiology
Time Factors
Treatment Outcome
Gut microbiota
Epilepsy
Cronobacter
Ketogenic diet
Seizures
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Summary:AIM To investigate whether patients with refractory epilepsy and healthy infants differ in gut microbiota(GM),and how ketogenic diet(KD) alters GM.METHODS A total of 14 epileptic and 30 healthy infants were recruited and seizure frequencies were recorded. Stool samples were collected for 16 S r DNA sequencing using the Illumina Miseq platform. The composition of GM in each sample was analyzed with MOTHUR,and intergroup comparison was conducted by R software.RESULTS After being on KD treatment for a week,64% of epileptic infants showed an obvious improvement,with a 50% decrease in seizure frequency. GM structure in epileptic infants(P1 group) differed dramatically from that in healthy infants(Health group). Proteobacteria,which had accumulated significantly in the P1 group,decreased dramatically after KD treatment(P2 group). Cronobacter predominated in the P1 group and remained at a low level both in the Health and P2 groups. Bacteroides increased significantly in the P2 group,in which Prevotella and Bifidobacterium also grew in numbers and kept increasing.CONCLUSION GM pattern in healthy infants differed dramatically from that of the epileptic group. KD could significantly modify symptoms of epilepsy and reshape the GM of epileptic infants.
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Author contributions: Dai WK designed the study and Wang WJ managed the project; Zhou Q and Qiu CZ interpreted the data; Xie G and Zhou Q wrote the manuscript; Qiu CZ and Li YH conducted bioinformatics analysis; Wang HP and Ye JH collected sample information; Liao JX, Lu XG, Lin SF and Ma ZY contributed to the study design and patients’ diagnoses; all authors read and approved the final manuscript. Xie G, Zhou Q and Qiu CZ contributed equally to this work.
Correspondence to: Wen-Jian Wang, Doctor, Director, Department of Respiratory Medicine, Shenzhen Children’s Hospital, No. 7019, Yitian Road, Shenzhen 518026, China. dhbk2005@163.com
Telephone: +86-755-83936101 Fax: +86-755-83009800
Supported by the Innovation Fund of Science and Technology Commission of Shenzhen Municipality, China, No. JCYJ20150403100317071.
ISSN:1007-9327
2219-2840
2219-2840
DOI:10.3748/wjg.v23.i33.6164