Protein Structure and Function Prediction Using I-TASSER

I-TASSER is a hierarchical protocol for automated protein structure prediction and structure-based function annotation. Starting from the amino acid sequence of target proteins, I-TASSER first generates full-length atomic structural models from multiple threading alignments and iterative structural...

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Bibliographic Details
Published inCurrent protocols in bioinformatics Vol. 52; p. 5.8.1
Main Authors Yang, Jianyi, Zhang, Yang
Format Journal Article
LanguageEnglish
Published United States 01.12.2015
Subjects
Algorithms
Amino Acid Sequence
Binding Sites
Computational Biology - methods
Databases, Protein
Gene Ontology
Ligands
Models, Molecular
Molecular Sequence Data
Protein Structure, Secondary
Protein Structure, Tertiary
Proteins - chemistry
Sequence Alignment
Solvents
protein function annotation
threading
protein structure prediction
I-TASSER
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Summary:I-TASSER is a hierarchical protocol for automated protein structure prediction and structure-based function annotation. Starting from the amino acid sequence of target proteins, I-TASSER first generates full-length atomic structural models from multiple threading alignments and iterative structural assembly simulations followed by atomic-level structure refinement. The biological functions of the protein, including ligand-binding sites, enzyme commission number, and gene ontology terms, are then inferred from known protein function databases based on sequence and structure profile comparisons. I-TASSER is freely available as both an on-line server and a stand-alone package. This unit describes how to use the I-TASSER protocol to generate structure and function prediction and how to interpret the prediction results, as well as alternative approaches for further improving the I-TASSER modeling quality for distant-homologous and multi-domain protein targets.
ISSN:1934-340X
DOI:10.1002/0471250953.bi0508s52