Secretory vesicles of immune cells contain only a limited number of interleukin 6 molecules

Immune cells communicate by releasing large quantities of cytokines. Although the mechanisms of cytokine secretion are increasingly understood, quantitative knowledge of the number of cytokines per vesicle is still lacking. Here, we measured with quantitative microscopy the release rate of vesicles...

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Bibliographic Details
Published inFEBS letters Vol. 592; no. 9; pp. 1535 - 1544
Main Authors Verboogen, Daniëlle R. J., Beest, Martin, Honigmann, Alf, Bogaart, Geert
Format Journal Article
LanguageEnglish
Published England John Wiley and Sons Inc 01.05.2018
Subjects
Cell Membrane - metabolism
dendritic cell
Dendritic Cells - cytology
exocytosis
Humans
interleukin 6
Interleukin-6 - metabolism
Protein Transport
Research Letter
Research Letters
Secretory Vesicles - metabolism
interleukin 6
exocytosis
dendritic cell
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Summary:Immune cells communicate by releasing large quantities of cytokines. Although the mechanisms of cytokine secretion are increasingly understood, quantitative knowledge of the number of cytokines per vesicle is still lacking. Here, we measured with quantitative microscopy the release rate of vesicles potentially carrying interleukin‐6 (IL‐6) in human dendritic cells. By comparing this to the total secreted IL‐6, we estimate that secretory vesicles contain about 0.5–3 IL‐6 molecules, but with a large spread among cells/donors. Moreover, IL‐6 did not accumulate within most cells, indicating that synthesis and not trafficking is the bottleneck for IL‐6 production. IL‐6 accumulated in the Golgi apparatus only in ~ 10% of the cells. Understanding how immune cells produce cytokines is important for designing new immunomodulatory drugs.
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Edited by Michael Bubb
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.13036