Green Production of Anionic Surfactant Obtained from Pea Protein

• Published in: Journal of surfactants and detergents Vol. 14; no. 4; pp. 535 - 544
• Main Authors: Rondel, Caroline, Portet, Bénédicte, Alric, Isabelle, Mouloungui, Zéphirin, Blanco, Jean-François, Silvestre, Françoise
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.10.2011; Springer Nature B.V; Springer Verlag
• Subjects: Amino acids; Aquatic Pollution; Chemical and Process Engineering; Chemistry; Chemistry and Materials Science; Engineering Sciences; Enzymatic hydrolysis; Foaming; Food engineering; Green chemistry; Hydrolysis; Industrial Chemistry/Chemical Engineering; Ions; Legumes; Life Sciences; Natural resources; N‐acylation; Original Article; Pea protein; Petrochemicals; Physical Chemistry; Polymer Sciences; Proteins; Raw materials; Renewable resources; Surfaces and Interfaces; Surfactant properties; Surfactants; Thin Films; Waste Water Technology; Water Management; Water Pollution Control; Surfactant properties; Green chemistry; Pea protein; acylation; Enzymatic hydrolysis; N-acylation; WHEY-PROTEIN; HYDROLYSIS; BIOSURFACTANTS; L; ISOLATE; FUNCTIONAL-PROPERTIES; PEPTIDES; SODIUM DODECYL-SULFATE; ELECTROPHORESIS; POLYACRYLAMIDE-GEL
• ISSN: 1097-3958; 1558-9293
• DOI: 10.1007/s11743-011-1283-2

A pea protein isolate was hydrolyzed by a double enzyme treatment method in order to obtain short peptide sequences used as raw materials to produce lipopeptides-based surfactants. Pea protein hydrolysates were prepared using the combination of Alcalase and Flavourzyme. The influence of the process variables was studied to optimize the proteolytic degradation to high degrees of hydrolysis. The average peptide chain lengths were obtained at 3–5 amino acid units after a hydrolysis of 30 min with the mixture of enzymes. Then, N -acylation in water, in presence of acid chloride (C12 and C16), carried out with a conversion rate of amine functions of 90%, allowed to obtain anionic surfactant mixtures (lipopeptides and sodium fatty acids). These two steps were performed in water, in continuous and did not generate any waste. This process was therefore in line with green chemistry principles. The surface activities (CMC, foaming and emulsifying properties) of these mixtures were also studied. These formulations obtained from natural renewable resources and the reactions done under environmental respect, could replace petrochemical based surfactants for some applications,.