Carbon nanotubes induce oxidative DNA damage in RAW 264.7 cells

• Published in: Environmental and molecular mutagenesis Vol. 51; no. 4; pp. 294 - 303
• Main Authors: Migliore, Lucia, Saracino, Doriana, Bonelli, Alessia, Colognato, Renato, D'Errico, Maria R, Magrini, Andrea, Bergamaschi, Antonio, Bergamaschi, Enrico
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2010; Wiley-Liss
• Subjects: Animals; Biological and medical sciences; Carbon; carbon nanotubes; Cell Line; comet assay; DNA Damage; DNA oxidative damage; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; genotoxicity; Medical sciences; Mice; micronucleus test; Mutagenicity Tests; Mutagens - toxicity; Nanotubes, Carbon - toxicity; Oxidative Stress - drug effects; RAW 264.7; Toxicology; Oxidative stress; Micronucleus test; DNA oxidative damage; Toxicity; Mutagenicity testing; Genotoxicity; Nanotube; RAW 264.7; Comet assay; Toxicology; carbon nanotubes; DNA; Genetics; Lesion
• ISSN: 0893-6692; 1098-2280
• DOI: 10.1002/em.20545

The induction of DNA and chromosome damage following in vitro exposure to carbon nanotubes (CNT) was assessed on the murine macrophage cell line RAW 264.7 by means of the micronucleus (MN) and the comet assays. Exposures to two CNT preparations (single-walled CNT (SWCNT > 90%) and multiwalled CNT (MWCNT > 90%) were performed in increasing mass concentrations (0.01-100 μg/ml). The frequency of micronuclei was significantly increased in cells treated with SWCNT (at doses above 0.1 μg/ml), whereas MWCNT had the same effect at higher concentrations (1 μg/ml) (P < 0.05). The results of the comet assay revealed that the effects of treatment with SWCNT were detectable at all concentrations tested (1-100 μg/ml); oxidized purines increased significantly, whereas pyrimidines showed a significant increase (P < 0.001) only at the highest concentration (100 μg/ml). In cells treated with MWCNT, an increase in DNA migration due to the oxidative damage to purines was observed at a concentration of 1 and 10 μg/ml, whereas pyrimidines showed a significant increase only at the highest mass concentration tested. However, both SWCNT and MWCNT induced a statistically significant cytotoxic effect at the highest concentrations tested (P < 0.001). These findings suggest that both the MN and comet assays can reliably detect small amount of damaged DNA at both chromosome and nuclear levels in RAW 264.7 cells. Moreover, the modified version of the comet assay allows the specific detection of the induction of oxidative damage to DNA, which may be the underlying mechanism involved in the CNT-associated genotoxicity. Environ. Mol. Mutagen., 2010.