rapid diagnostic technique of Bactrocera cucurbitae and Bactrocera zonata (Diptera: Tephritidae) for quarantine application
BACKROUND: The melon fruit fly Bactrocera cucurbitae and the peach fruit fly Bactrocera zonata are serious pests, native to Asia, that have recently invaded Egypt. In Tunisia, no report of these species has yet been made, but pest risk data suggest that both Bactrocera species are likely to establis...
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Published in | Pest management science Vol. 69; no. 6; pp. 744 - 746 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
01.06.2013
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | BACKROUND: The melon fruit fly Bactrocera cucurbitae and the peach fruit fly Bactrocera zonata are serious pests, native to Asia, that have recently invaded Egypt. In Tunisia, no report of these species has yet been made, but pest risk data suggest that both Bactrocera species are likely to establish in other countries of the Mediterranean region. RESULTS: A quick method, based on a PCR‐RFLP of the mitochondrial COI gene, has been developed to enhance species identification for quarantine purposes. The restriction enzymes DdeI and XmnI were used to digest COI PCR products, enabling a rapid diagnosis of B. cucurbitae and B. zonata and their distinction from Tunisia's most devastating fruit pest, Ceratitis capitata. CONCLUSION: The simplicity and relatively low cost of this molecular approach will replace the need to rear immature stages through to adults for identification and will facilitate rapid quarantine decisions providing greater plant protection. |
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Bibliography: | http://dx.doi.org/10.1002/ps.3433 ark:/67375/WNG-0M7Q6TZM-4 ArticleID:PS3433 istex:F5AA0C8EEC53A4361FC00E3244DA03838FFF48E3 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1526-498X 1526-4998 |
DOI: | 10.1002/ps.3433 |