Quantitative CARS Molecular Fingerprinting of Single Living Cells with the Use of the Maximum Entropy Method

Fast CARS: The cell‐death process was observed in real time at the subcellular level by coherent anti‐Stokes Raman microspectroscopy, an extension of anti‐Stokes Raman scattering (CARS) microscopy. Changes in the chemical contrast during the dying process can be clearly resolved (see picture).

Saved in:
Bibliographic Details
Published inAngewandte Chemie (International ed.) Vol. 49; no. 38; pp. 6773 - 6777
Main Authors Okuno, Masanari, Kano, Hideaki, Leproux, Philippe, Couderc, Vincent, Day, James P.R, Bonn, Mischa, Hamaguchi, Hiro-o
Format Journal Article
LanguageEnglish
Published Weinheim Wiley-VCH Verlag 10.09.2010
WILEY-VCH Verlag
WILEY‐VCH Verlag
Subjects
CARS (coherent anti-Stokes Raman scattering)
Coloring Agents - chemistry
Entropy
Saccharomycetales - chemistry
Saccharomycetales - cytology
Spectrum Analysis, Raman - methods
time-resolved spectroscopy
Vibration
vibrational spectroscopy
Online AccessGet full text

Cover

More Information
Summary:Fast CARS: The cell‐death process was observed in real time at the subcellular level by coherent anti‐Stokes Raman microspectroscopy, an extension of anti‐Stokes Raman scattering (CARS) microscopy. Changes in the chemical contrast during the dying process can be clearly resolved (see picture).
Bibliography:http://dx.doi.org/10.1002/anie.201001560
JST
ArticleID:ANIE201001560
This work was supported by the SENTAN project (Program-S) of the Japan Science and Technology Agency (JST). H. Kano gratefully acknowledges financial support from the Precursory Research for Embryonic Science and Technology (PRESTO) program of JST. The authors thank C. Onogi for providing the spontaneous Raman spectrum of yeast mitochondria, Leukos and Horus Laser companies for technical support, and Dr. F. Omura and H. Yomo (Suntory Co., Ltd.) for providing us with the yeast sample. We gratefully acknowledge J. Ukon (HORIBA, Ltd.) for assisting in the collaboration between the Japanese and French groups.
Japan Science and Technology Agency
istex:93C4423BFFE3E2F6AD55E4C52E51126BCA12A48E
ark:/67375/WNG-5L3JXP37-P
This work was supported by the SENTAN project (Program‐S) of the Japan Science and Technology Agency (JST). H. Kano gratefully acknowledges financial support from the Precursory Research for Embryonic Science and Technology (PRESTO) program of JST. The authors thank C. Onogi for providing the spontaneous Raman spectrum of yeast mitochondria, Leukos and Horus Laser companies for technical support, and Dr. F. Omura and H. Yomo (Suntory Co., Ltd.) for providing us with the yeast sample. We gratefully acknowledge J. Ukon (HORIBA, Ltd.) for assisting in the collaboration between the Japanese and French groups.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201001560