Molecular Analysis of Malassezia Microflora in Seborrheic Dermatitis Patients: Comparison with Other Diseases and Healthy Subjects

• Published in: Journal of investigative dermatology Vol. 128; no. 2; pp. 345 - 351
• Main Authors: Tajima, Mami, Sugita, Takashi, Nishikawa, Akemi, Tsuboi, Ryoji
• Format: Journal Article
• Language: English
• Published: Danvers, MA Elsevier Inc 01.02.2008; Nature Publishing; Elsevier Limited
• Subjects: Adult; Aged; Biological and medical sciences; Dermatitis, Atopic - microbiology; Dermatitis, Seborrheic - microbiology; Dermatology; Dermatomycoses - microbiology; DNA, Fungal - analysis; Female; Genotype; Humans; Malassezia - genetics; Malassezia - isolation & purification; Male; Medical sciences; Middle Aged; Plasmids; Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Skin involvement in other diseases. Miscellaneous. General aspects; Tinea Versicolor - microbiology; Human; Skin disease; Healthy subject; Disease; Dermatology; Seborrheic dermatitis; Microflora; Sebaceous gland; Fungi; Malassezia; Fungi Imperfecti; Molecular biology; Comparative study
• ISSN: 0022-202X; 1523-1747; 1523-1747
• DOI: 10.1038/sj.jid.5701017

Malassezia species colonize the skin of normal and various pathological conditions including pityriasis versicolor (PV), seborrhoeic dermatitis (SD) and atopic dermatitis (AD). To elucidate the pathogenic role of Malassezia species in SD, Malassezia microflora of 31 Japanese SD patients was analyzed using a PCR-based, culture-independent method. Nested PCR assay using the primers in the rRNA gene indicated that the major Malassezia species in SD were M. globosa and M. restricta, found in 93 and 74% of the patients, respectively. The detection rate and number of each species varied similarly in SD, PV and healthy subjects (HSs), whereas AD showed higher values. Real-time PCR assay showed that the lesional skin harbored approximately three times the population of genus Malassezia found in nonlesional skin (P<0.05), and that M. restricta is a significantly more common species than M. globosa in SD (P<0.005). Genotypic analysis of the rRNA gene showed that the M. globosa and M. restricta from SD patients fell into specific clusters, and could be distinguished from those collected from HSs, but not from those colleted from AD patients. Our results indicate that certain strains of M. restricta occur in the lesional skin of SD patients.