Human POT1 Facilitates Telomere Elongation by Telomerase
Mammalian telomeric DNA is mostly composed of double-stranded 5′-TTAGGG-3′ repeats and ends with a single-stranded 3′ overhang. Telomeric proteins stabilize the telomere by protecting the overhang from degradation [1, 2] or by remodeling the telomere into a T loop structure [3]. Telomerase is a ribo...
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Published in | Current biology Vol. 13; no. 11; pp. 942 - 946 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Inc
27.05.2003
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Subjects | |
Online Access | Get full text |
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Summary: | Mammalian telomeric DNA is mostly composed of double-stranded 5′-TTAGGG-3′ repeats and ends with a single-stranded 3′ overhang. Telomeric proteins stabilize the telomere by protecting the overhang from degradation [1, 2] or by remodeling the telomere into a T loop structure [3]. Telomerase is a ribonucleoprotein that synthesizes new telomeric DNA [4, 5]. In budding yeast, other proteins, such as Cdc13p, that may help maintain the telomere end by regulating the recruitment or local activity of telomerase have been identified [6–9]. Pot1 is a single-stranded telomeric DNA binding protein first identified in fission yeast, where it was shown to protect telomeres from degradation [10]. Human POT1 (hPOT1) protein is known to bind specifically to the G-rich telomere strand [11]. We now show that hPOT1 can act as a telomerase-dependent, positive regulator of telomere length. Three splice variants of hPOT1 were overexpressed in a telomerase-positive human cell line. All three variants lengthened telomeres, and splice variant 1 was the most effective. hPOT1 was unable to lengthen the telomeres of telomerase-negative cells unless telomerase activity was induced. These data suggest that a normal function of hPOT1 is to facilitate telomere elongation by telomerase. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0960-9822 1879-0445 |
DOI: | 10.1016/S0960-9822(03)00339-7 |