Expression of USP15, TβR- Ⅰ and Smad7 in Psoriasis

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 3; pp. 415 - 419
• Main Author: 冯爱平 和义敏 刘欣欣 李家文 涂亚庭 胡枫 陈善娟
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.06.2014; Department of Dermatology, Union Hospital Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
• Subjects: Adult; Cell Line; Female; Gene Expression; HaCaT细胞; Humans; Immunohistochemistry; Keratinocytes - cytology; Keratinocytes - metabolism; Male; Medicine; Medicine & Public Health; Middle Aged; mRNA表达; Protein-Serine-Threonine Kinases - biosynthesis; Protein-Serine-Threonine Kinases - genetics; Psoriasis - genetics; Psoriasis - metabolism; Receptors, Transforming Growth Factor beta - biosynthesis; Receptors, Transforming Growth Factor beta - genetics; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; Signal Transduction - genetics; siRNA; Skin - metabolism; Smad7; Smad7 Protein - biosynthesis; Smad7 Protein - genetics; Ubiquitin-Specific Proteases - biosynthesis; Ubiquitin-Specific Proteases - genetics; Young Adult; 信号转导通路; 免疫组织化学染色; 牛皮癣; 转化生长因子; USP15; psoriasis; TβR-I; Smad7; TβR-
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1293-1

Summary： The deubiquitinating enzyme ubiquitin specific peptidase 15 （USP15） is regarded as a regulator of TGFβ signaling pathway. This process depends on Smad7, the inhibitory factor of the TGFβ signal, and type Ⅰ TGFβ receptor （TβR- Ⅰ ）, one of the receptors of TGFβ. The expression level of USP 15 seems to play vital roles in the pathogenesis of many neoplasms, but so far there has been no report about USP15 in psoriasis. In this study, immunohistochemical staining of USP15, TβR- Ⅰ and Smad7 was performed in 30 paraffin-embedded psoriasis specimens and 10 normal specimens to investigate the expression of USP15, TβR- Ⅰ and Smad7 in psoriasis and to explore the relevance among them. And USP 15 small interfering RNA （USP 15 siRNA） was used to transfect Hacat cells to detect the mRNA expression of TβR- Ⅰ and Smad7. Of 30 cases of psoriasis in active stage, 28, 24 and 26 cases were positive for USP15, TβR- Ⅰ and Smad7 staining, respectively. The positive rates of USP15 and Smad7 were significantly higher in psoriasis specimens than in normal skin specimens （44.1%±26.0% vs. 6.1%±6.6%, 47.2%±27.1% vs. 6.6%±7.1%）, and positive rate of TβR- I （20.3%±22.2%） in psoriasis was lower than that in normal skin specimens （46.7%±18.2%）. There was a significant positive correlation between USP15 and Smad7 expression, and significant negative correlations between USP15 and TβR- Ⅰ expression, and between TβR- Ⅰ and Smad7 expression in psoriasis. After transfection of USP15 siRNA in Hacat ceils, the expression ofTβR- Ⅰ mRNA was up-regulated and that of Smad7 was down-regulated. It is concluded that USP15 may play a role in the pathogenesis of psoriasis through regulating the TβR- Ⅰ/Smad7 pathway and there may be other cell signaling pathways interacting with USP 15 to take part in the development of psoriasis.