Characterization and Functional Analysis of a Novel Fungal Immunomodulatory Protein Gene from Ganoderma leucocontextum in B16-F10 Mouse Melanoma Cells

• Published in: International journal of molecular sciences Vol. 26; no. 11; p. 5063
• Main Authors: Yang, Jiayi, Jin, Mengyuan, Zhang, Lida, Wu, Yingying, Zhou, Xuanwei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.05.2025; MDPI
• Subjects: Amino acids; Animals; Apoptosis; Apoptosis - drug effects; Cancer; Cell cycle; Cell growth; Cell Line, Tumor; Cell Proliferation - drug effects; Cloning; Cytokines; Free radicals (Chemistry); Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungal Proteins - pharmacology; Fungi; Ganoderma - genetics; Ganoderma - metabolism; Genes; Immunologic Factors - genetics; Immunologic Factors - pharmacology; Melanins - biosynthesis; Melanins - metabolism; Melanoma; Melanoma, Experimental - drug therapy; Melanoma, Experimental - metabolism; Melanoma, Experimental - pathology; Mice; Molecular weight; Phosphorylation; Proteins; Recombinant Proteins - genetics; Recombinant Proteins - pharmacology; Telomerase; Transcription factors; China; fungal immunomodulatory proteins; Ganoderma leucocontextum; B16-F10 melanoma cell; protein expression
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms26115063

Ganoderma leucocontextum, a newly identified species from the Tibetan Plateau, has been mainly studied for its polysaccharides and triterpenoids, with no prior reports on fungal immunomodulatory proteins (FIPs). This study explores the biological activity of FIP-gle2, cloned from G. leucocontextum and expressed in Pichia pastoris. The effects and mechanisms of recombinant FIP-gle2 (rFIP-gle2) on cell activity and melanin synthesis in mouse melanoma B16-F10 cells were investigated in vitro. The results showed that the FIP-gle2 gene, with an open reading frame (ORF) of 333 bp, encodes a 111-amino acid polypeptide with a molecular weight of 12.60 kDa and an isoelectric point of 4.48. We achieved a yield of 184.18 mg/L of rFIP-gle2. In vitro functional experiments showed that rFIP-gle2 significantly inhibited the proliferation of B16-F10 melanoma cells and induced apoptosis in a dose-dependent manner, particularly at concentrations above 1 μg/mL. At 3 μg/mL, rFIP-gle2 effectively inhibited tyrosinase activity and reduced melanin content, downregulating microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), and tyrosinase-related proteins (TRP-1 and TRP-2). Furthermore, RNA-seq analysis indicated that differentially expressed genes in treated cells were enriched in the mitogen-activated protein kinase (MAPK) signaling pathway, with Western blotting confirming enhanced phosphorylation of JNK, ERK, and p38 proteins. Thus, P. pastoris is an effective host for rFIP-gle2 production, which shows potential for applications in pharmaceuticals, cosmeceuticals, and food fields.