Gene Chip Technology Used in the Detection of HPV Infection in Esophageal Cancer of Kazakh Chinese in Xinjiang Province

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 3; pp. 343 - 347
• Main Author: 陈卫刚 杨春梅 徐丽红 张宁 刘晓燕 马云贵 霍晓玲 韩玉胜 田德安 郑勇
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.06.2014; Department of Gastroenterology, Tongji Hospital Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Department of Gastroenterology, First Affiliated Hospital of the Medical College, Shihezi University, Shihezi 832002, China%Department of Gastroenterology, First Affiliated Hospital of the Medical College, Shihezi University, Shihezi 832002, China%Hospital of Xinyuan County, Yili 835800, China%The Hospital of No.4 Agricultural Division, The Xinjiang Production and Construction Corps 835000, China
• Subjects: Aged; Asian Continental Ancestry Group; Carcinoma, Squamous Cell - ethnology; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - virology; Case-Control Studies; China; DNA, Neoplasm - analysis; DNA, Neoplasm - genetics; DNA, Viral - analysis; DNA, Viral - genetics; Esophageal Neoplasms - ethnology; Esophageal Neoplasms - genetics; Esophageal Neoplasms - virology; Female; Host-Pathogen Interactions - genetics; HPV16; Human papillomavirus 16; Human papillomavirus 16 - genetics; Human papillomavirus 18 - genetics; Humans; Male; Medicine; Medicine & Public Health; Middle Aged; Molecular Typing - methods; Molecular Typing - statistics & numerical data; Oligonucleotide Array Sequence Analysis - methods; Papillomaviridae - classification; Papillomaviridae - genetics; Papillomaviridae - physiology; Papillomavirus Infections - ethnology; Papillomavirus Infections - genetics; Papillomavirus Infections - virology; Polymerase Chain Reaction; 乳头状瘤病毒; 基因芯片技术; 新疆; 检测; 病毒感染; 食管癌; 鳞状细胞癌; China; gene chip; esophageal squamous cell carcinoma; Kazakh; HPV
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1280-6

Summary： This study was aimed to screen human papillomavirus （HPV） types associated with esophageal squamous cell carcinoma of Kazakh in Xinjiang using the gene chip technique and study the clinical significance of this application. The DNAs were collected from esophageal squamous cell carcinoma tissues and healthy esophageal mucosa of Kazakh adults in Xinjiang, and amplified firstly using HPV MY09/11 and then using HPV G5＋/6＋ to screen positive HPV specimens. These positive specimens were further detected by the gene chip technique to screen highly pathogenic HPV types. After determination with nested PCR amplification with HPV MY09/ll and G5＋/6＋, the infection rate of HPV was 66.67% in the esophageal squamous cell carcinoma group and 12.12% in the healthy control group. By testing the positive HPV specimens from the esophageal squamous cell carcinoma group, the infection rate of HPV16 was 97.72% and the co-infection rate of HPV16 and HPV18 was 2.27%. HPV16 infection may be involved in the development of esophageal squamous cell carcinoma in Xinjiang Hazakh adults.