Identification of a Gene (GPR30) with Homology to the G-Protein-Coupled Receptor Superfamily Associated with Estrogen Receptor Expression in Breast Cancer

• Published in: Genomics (San Diego, Calif.) Vol. 45; no. 3; pp. 607 - 617
• Main Authors: Carmeci, Charles, Thompson, Devon A., Ring, Huijun Z., Francke, Uta, Weigel, Ronald J.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.11.1997; Elsevier
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Breast Neoplasms - genetics; Carcinoma - genetics; Cell physiology; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Chromosome Mapping; Cricetinae; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Neoplastic; Genetic Techniques; GTP-Binding Proteins - metabolism; Humans; Molecular and cellular biology; Molecular Sequence Data; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Receptors, Estrogen - genetics; Receptors, Estrogen - metabolism; Receptors, G-Protein-Coupled; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Tissue Distribution; Tumor Cells, Cultured; Genetic mapping; Human; Nucleotide sequence; Estrogen; Homology; Chromosome C7; Gene expression; Mammary gland diseases; Signal transduction; Cell line; Gene; Multigene family; Tumor; Mammary gland; Biological receptor; Hormonal receptor
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1997.4972

Using the technique of differential cDNA library screening, a cDNA clone was isolated from an estrogen receptor (ER)-positive breast carcinoma cell line (MCF7) cDNA library based upon the overexpression of this gene compared to an ER-negative cell line (MDA-MB-231). Sequence analysis of this clone determined that it shared significant homology to G-protein-coupled receptors. This receptor, GPCR-Br, was abundantly expressed in the ER-positive breast carcinoma cell lines MCF7, T-47D, and MDA-MB-361. Expression was absent or minimal in the ER-negative breast carcinoma cell lines BT-20, MDA-MB-231, and HBL-100. GPCR-Br was ubiquitously expressed in human tissues examined but was most abundant in placenta. GPCR-Br expression was examined in 11 primary breast carcinomas. GPCR-Br was detected in all 4 ER-positive tumors and only 1 of 7 ER-negative tumors. Based upon PCR analysis in hybrid cell lines, the gene for GPCR-Br (HGMW-approved symbol GPR30) was mapped to chromosome 7p22. The pattern of expression of GPCR-Br indicates that this receptor may be involved in physiologic responses specific to hormonally responsive tissues.