Comparative Pathogenicity of Lomentospora prolificans (Scedosporium prolificans) Isolates from Mexican Patients

• Published in: Mycopathologia (1975) Vol. 182; no. 7-8; pp. 681 - 689
• Main Authors: Elizondo-Zertuche, Mariana, Montoya, Alexandra M., Robledo-Leal, Efrén, Garza-Veloz, Idalia, Sánchez-Núñez, Ana L., Ballesteros-Elizondo, Raquel, González, Gloria M.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2017; Springer; Springer Nature B.V
• Subjects: Adolescent; Adult; Aged; Amphotericin B; Animal Structures - microbiology; Animals; Antifungal Agents - pharmacology; Biomedical and Life Sciences; Brain; Caspofungin; Child; Cluster Analysis; Colony Count, Microbial; Disease Models, Animal; Disseminated infection; DNA, Fungal - chemistry; DNA, Fungal - genetics; DNA, Ribosomal Spacer - chemistry; DNA, Ribosomal Spacer - genetics; Echinocandins; Eukaryotic Microbiology; Female; Fluconazole; Fungi; Fungicides; Health aspects; Humans; Immunocompetence; Infection; Infections; Kidneys; Life Sciences; Male; Medical Microbiology; Mexico; Micafungin; Mice; Mice, Inbred ICR; Microbial Ecology; Microbial Sensitivity Tests; Microbiology; Middle Aged; Minimum inhibitory concentration; Mycoses - microbiology; Pathogenicity; Pathogens; Phylogeny; Plant Sciences; Posaconazole; Quality control; Ribosomal DNA; Scedosporium - classification; Scedosporium - genetics; Scedosporium - isolation & purification; Scedosporium - pathogenicity; Sequence Analysis, DNA; Spacer; Spleen; Strain; Survival; Survival Analysis; Transplants & implants; Voriconazole; Pathogenicity; In vitro susceptibility; Survival study; Tissue burden; Lomentospora prolificans
• ISSN: 0301-486X; 1573-0832
• DOI: 10.1007/s11046-017-0137-5

We identified 11 Lomentospora prolificans isolates recovered from Mexican patients using phenotypic and molecular characteristics. The identification of isolates was assessed by internal transcribed spacer (ITS rDNA) sequencing. In vitro susceptibility to amphotericin B, fluconazole, voriconazole, posaconazole, caspofungin, anidulafungin and micafungin was determined according to Clinical and Laboratory Standards Institute (CLSI) procedures. Three isolates (07-2239, 11-2242 and 04-2673) were used to induce systemic infection in immunocompetent ICR mice. Survival and tissue burden studies were used as markers of pathogenicity. All of the strains were resistant to every antifungal tested with MIC’s for AmB (8–>8 µg/ml), VRC (16–>16 µg/ml), PSC (16–>16 µg/ml), FLC (64–>64 µg/ml) and echinocandins with MICs ≥8 µg/ml. One hundred, ninety and sixty percent of the infected mice with the strains 07-2239, 11-2242 and 04-2673 died during the study, respectively. Regarding tissue burden, the highest fungal load of the infected mice was detected in brain followed by spleen and kidney, regardless of the strain.