Ameliorating Effects of Fermented Rice Bran Extract on Oxidative Stress Induced by High Glucose and Hydrogen Peroxide in 3T3-L1 Adipocytes

• Published in: Plant foods for human nutrition (Dordrecht) Vol. 66; no. 3; pp. 285 - 290
• Main Authors: Kim, Dongyeop, Han, Gi Dong
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.09.2011; Springer
• Subjects: 3T3-L1 Cells; Adipocytes - drug effects; Adipocytes - metabolism; Adiponectin - metabolism; Animals; Biological and medical sciences; Biological Transport - drug effects; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Ecology; Fat industries; Fermentation; Food industries; Food Science; Fundamental and applied biological sciences. Psychology; Glucose - adverse effects; Glucose Transporter Type 4 - metabolism; Hydrogen Peroxide; Insulin - metabolism; Insulin Resistance; Mice; Nutrition; Original Paper; Oryza; Oxidative Stress - drug effects; Phenols - pharmacology; Phenols - therapeutic use; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant Physiology; PPAR gamma - metabolism; Reactive Oxygen Species - metabolism; Seeds; Up-Regulation; Fermented rice bran; Oxidative stress; Insulin resistance; Reactive oxygen species; 3T3-L1 adipocytes; Adipocyte; Oxygen; Hydrogen; Staining; Extract; Glucose; Fermented product; Red; Oil; Carbohydrate; Peroxides; Species; Rice bran
• ISSN: 0921-9668; 1573-9104
• DOI: 10.1007/s11130-011-0243-3

In this study, we investigated whether fermented rice bran (FRB) can ameliorate the oxidative stress induced by high glucose and hydrogen peroxide (H 2 O 2 ) in 3T3-L1 adipocytes by analyzing reactive oxygen species (ROS), oil red O staining, as well as the expression of mRNAs related to glucose homeostasis and adipogenesis. It was first confirmed that rice bran fermented by Issatchenkia orientalis MFST1 extract increased free phenolic content compared to non-fermented rice bran. The FRB extract strongly inhibited ROS generation and upregulated the expression of PPAR-γ and adiponectin. Moreover, FRB upregulated GLUT4 related to glucose transportation and insulin sensitivity. Taken together, FRB extract ameliorated oxidative stress-induced insulin resistance by neutralizing free radicals and upregulating adiponectin in adipocytes. Our results provide information toward understanding the beneficial effects of FRB on oxidative stress.