Meiotic Recombination Remote from Prominent DNA Break Sites in S. pombe
DNA breakage is intimately associated with meiotic recombination in the fission yeast Schizosaccharomyces pombe. Sites of prominent DNA breakage were found ∼25 to ∼200 kb apart in the genomic regions surveyed. We examined in detail a 501 kb region of chromosome I and found six sites, or tight cluste...
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Published in | Molecular cell Vol. 9; no. 2; pp. 253 - 263 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.02.2002
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Subjects | |
Online Access | Get full text |
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Summary: | DNA breakage is intimately associated with meiotic recombination in the fission yeast
Schizosaccharomyces pombe. Sites of prominent DNA breakage were found ∼25 to ∼200 kb apart in the genomic regions surveyed. We examined in detail a 501 kb region of chromosome I and found six sites, or tight clusters of sites, at which ∼2%–11% of the DNA accumulated breaks in a
rad50S mutant. In contrast to the discrete, widely spaced distribution of prominent break sites, recombination in this region was more uniformly distributed (0.7–1.6 cM/10 kb) whether the genetic interval tested contained no, one, or more such sites. We infer that although recombination depends upon DNA breakage, recombination often occurs remote from these sites (tens of kilobases away); we discuss mechanisms by which this may occur. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1097-2765 1097-4164 |
DOI: | 10.1016/S1097-2765(02)00452-5 |