Murine cerebellar neurons express a novel gene encoding a protein related to cell cycle control and cell fate determination proteins

• Published in: The Journal of biological chemistry Vol. 269; no. 13; pp. 9946 - 9951
• Main Authors: TAOKA, M, ISOBE, T, SI-YOUNG SONG, YAMAKUNI, T, OKUYAMA, T, WATANABE, M, KONDO, H, YAMAKAWA, Y, OZAWA, F, HISHINUMA, F, KUBOTA, M, MINEGISHI, A
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 01.04.1994
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Animals; Base Sequence; Biological and medical sciences; Blotting, Northern; Brain - metabolism; Cell Cycle - genetics; Cerebellum - cytology; Cerebellum - metabolism; Chromatography, High Pressure Liquid; Cloning, Molecular; Consensus Sequence; DNA Primers; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene Library; In Situ Hybridization; Intercellular Signaling Peptides and Proteins; Mice; Mice, Neurologic Mutants; Miscellaneous; Molecular Sequence Data; Molecular Weight; Nerve Tissue Proteins - biosynthesis; Nerve Tissue Proteins - isolation & purification; Nerve Tissue Proteins - metabolism; Neurons - cytology; Neurons - metabolism; Oligonucleotide Probes; Polymerase Chain Reaction; Proteins; Purkinje Cells - cytology; Purkinje Cells - metabolism; Rats; Rats, Wistar; Reference Values; Repetitive Sequences, Nucleic Acid; Restriction Mapping; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; RNA, Messenger - metabolism; Sequence Homology, Amino Acid; Proteins; Cerebellum; Vertebrata; Mammalia; Granule neuron; Cell cycle; Primary structure; Molecular cloning; Cell differentiation; Gene expression
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(17)36974-0

We cloned cDNAs of a novel protein (designated V-1) that has been identified from among the developmentally regulated proteins in the rat cerebellum. Protein sequencing analysis (Taoka, M., Yamakuni, T., Song, S.-Y., Yamakawa, Y., Seta, K., Okuyama, T., and Isobe, T. (1992) Eur. J. Biochem. 207, 615-620) and cDNA sequence analysis revealed that the V-1 protein consists of 117 amino acids and contains 2.5 contiguous repeats of the cdc10/SWI6 motif, which was originally found in the products of the cell cycle control genes of yeasts and the cell fate determination genes in Drosophila and Caenorhabditis elegans. In situ hybridization histochemistry revealed that the expression of the V-1 gene is transiently increased in postmigratory granule cells during postnatal rat cerebellar development and thereafter is markedly suppressed, whereas Purkinje cells constitutively express V-1 mRNA. In contrast, cerebellar granule cells of the staggerer mutant mouse continue to express the V-1 gene even when the granule cells of the normal mouse have ceased to express the V-1 gene, suggesting that the expression of the V-1 gene in granule cells is regulated through the interaction with Purkinje cells. On the basis of these results, we postulate that the V-1 protein has a potential role in the differentiation of granule cells.