The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells

Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block–poly 2-(dimethylamino)ethyl metha...

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Published inFrontiers in pharmacology Vol. 10; p. 1194
Main Authors Cao, Zhonglin, Xiao, Huiyu, Li, Li, Liu, Maixian, Lin, Guimiao, Zhai, Peng, Yong, Ken-Tye, Wang, Xiaomei, Xu, Gaixia
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 10.10.2019
Subjects
gene carrier
hepatoma cancer cell
micelle
Pharmacology
QDs
siRNA
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Summary:Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block–poly 2-(dimethylamino)ethyl methacrylate (PCL-PDEM) cationic copolymer was developed to carry short interfering RNA (siRNA) for silencing interleukin 8 (IL-8) gene in hepatoma cancer cells. The transfection efficiency of the PCL-PDEM-siRNA/quantum dots (QDs) nanoplex has reached about 70%, and the expression level of IL-8 decreased about 63%. Furthermore, the codelivery of QDs and siRNA has been realized, which is beneficial to visualize the process of siRNA delivery. No considerable cytotoxicity from the nanoparticles has been observed, indicating that our responsive cationic micelle is potential in clinical trial for hepatoma cancer therapy.
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Edited by: Jiangjiang Qin, Zhejiang Chinese Medical University, China
This article was submitted to Pharmacology of Anti-Cancer Drugs, a section of the journal Frontiers in Pharmacology
Reviewed by: Supriya Dinkar Mahajan, University at Buffalo, United States; Jessica Reynolds, University at Buffalo, United States; Peihong Ni, Soochow University, China
ISSN:1663-9812
1663-9812
DOI:10.3389/fphar.2019.01194