Efficient Regulation of CO2 Assimilation Enables Greater Resilience to High Temperature and Drought in Maize
Increasing temperatures and extended drought episodes are among the major constraints affecting food production. Maize has a relatively high temperature optimum for photosynthesis compared to C 3 crops, however, the response of this important C 4 crop to the combination of heat and drought stress is...
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Published in | Frontiers in plant science Vol. 12; p. 675546 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
26.07.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Increasing temperatures and extended drought episodes are among the major constraints affecting food production. Maize has a relatively high temperature optimum for photosynthesis compared to C
3
crops, however, the response of this important C
4
crop to the combination of heat and drought stress is poorly understood. Here, we hypothesized that resilience to high temperature combined with water deficit (WD) would require efficient regulation of the photosynthetic traits of maize, including the C
4
–CO
2
concentrating mechanism (CCM). Two genotypes of maize with contrasting levels of drought and heat tolerance, B73 and P0023, were acclimatized at high temperature (38°C versus 25°C) under well-watered (WW) or WD conditions. The photosynthetic performance was evaluated by gas exchange and chlorophyll
a
fluorescence, and
in vitro
activities of key enzymes for carboxylation (phosphoenolpyruvate carboxylase), decarboxylation (NADP-malic enzyme), and carbon fixation (Rubisco). Both genotypes successfully acclimatized to the high temperature, although with different mechanisms: while B73 maintained the photosynthetic rates by increasing stomatal conductance (gs), P0023 maintained gs and showed limited transpiration. When WD was experienced in combination with high temperatures, limited transpiration allowed water-savings and acted as a drought stress avoidance mechanism. The photosynthetic efficiency in P0023 was sustained by higher phosphorylated PEPC and electron transport rate (ETR) near vascular tissues, supplying chemical energy for an effective CCM. These results suggest that the key traits for drought and heat tolerance in maize are limited transpiration rate, allied with a synchronized regulation of the carbon assimilation metabolism. These findings can be exploited in future breeding efforts aimed at improving maize resilience to climate change. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Samuel Cordeiro Vitor Martins, Universidade Federal de Viçosa, Brazil; Nelson Facundo Rodriguez Lòpez, Universidad Industrial de Santander, Colombia Edited by: Fabricio Eulalio Leite Carvalho, Corporacion Colombiana de Investigacion Agropecuaria (Agrosavia) - CI La Suiza, Colombia This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2021.675546 |