Single Nucleotide Polymorphisms in Starch Biosynthetic Genes Associated With Increased Resistant Starch Concentration in Rice Mutant

• Published in: Frontiers in genetics Vol. 10
• Main Authors: Gurunathan, Selvakumar, Ramadoss, Bharathi Raja, Mudili, Venkataramana, Siddaiah, Chandranayaka, Kalagatur, Naveen Kumar, Bapu, Jutti Rajendran Kannan, Mohan, Chakrabhavi Dhananjaya, Alqarawi, Abdulaziz A., Hashem, Abeer, Abd_Allah, Elsayed Fathi
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 15.11.2019
• Subjects: amylose; Genetics; mutation/variants; resistant starch; rice; starch biosynthesis
• ISSN: 1664-8021; 1664-8021
• DOI: 10.3389/fgene.2019.00946

Resistant Starch (RS), plays a crucial role in human health and nutrition by controlling glucose metabolism. RS or dietary fibre content in rice is low because it goes through a variety of process before it is ready for cooking and consumption. Hence, this study was carried out to develop a rice mutant with increased RS. The rice mutant (γ278) with increased RS was developed by utilizing gamma (γ) rays as a mutagen. Mutant γ278 was characterized for mutations in the starch biosynthetic genes viz ., GBSSI, SSI, SSIIa, SSIIIa, SBEIa , and SBEIIb to reveal the functional mutations/variations led to high RS content in rice. A total of 31 sequence variants/mutations in six genes were identified. We report the discovery of three deleterious mutation/variants each in GBSSI, SSIIa , and SSIIIa with the potential to increase RS content in rice. Further, wild × mutant crosses were made to develop an F 2 population to study the effect of combination of deleterious mutations. The SNP ( GBSSI : ssIIa : ssIIIa ) combination responsible for high RS content in F 2 population was identified and recorded highest amylose content (AC) (26.18%) and RS (8.68%) content. In conclusion, this marker combination will be highly useful to develop a rice variety with increased RS.