Recombinant protein secretion in Pseudozyma flocculosa and Pseudozyma antarctica with a novel signal peptide

• Published in: Bioscience, biotechnology, and biochemistry Vol. 72; no. 12; pp. 3158 - 3166
• Main Authors: Cheng, Y.(Laval Univ., Quebec (Canada)), Avis, T.J, Bolduc, S, Zhao, Y, Anguenot, R, Neveu, B, Labbe, C, Belzile, F, Belanger, R.R
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.12.2008; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Amino Acid Sequence; BASIDIOMYCOTINA; Biological and medical sciences; cell processing; CLONACION; CLONAGE; CLONING; Fundamental and applied biological sciences. Psychology; Fungal Proteins - analysis; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Genes, Fungal; Genes, Reporter; Genome, Fungal; Genomics; Green Fluorescent Proteins - metabolism; LEVADURA; LEVURE; Molecular Sequence Data; PEPTIDE; PEPTIDES; PEPTIDOS; preprosequence; protein secretion; Protein Sorting Signals - genetics; PROTEINAS RECOMBINANTES; PROTEINE RECOMBINANTE; Pseudozyma; Pseudozyma antarctica; RECOMBINANT PROTEINS; Recombinant Proteins - analysis; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; SECRECION; SECRETION; SENALES; SIGNAL; signal peptide; SIGNALS; Ustilaginales - cytology; Ustilaginales - genetics; Ustilaginales - metabolism; YEASTS; signal peptide; Leader peptide; cell processing; Secretion; Pseudozyma; Recombinant protein; preprosequence; protein secretion
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.80340

Secretion of recombinant proteins aims to reproduce the correct posttranslational modifications of the expressed protein while simplifying its recovery. In this study, secretion signal sequences from an abundantly secreted 34-kDa protein (P34) from Pseudozyma flocculosa were cloned. The efficiency of these sequences in the secretion of recombinant green fluorescent protein (GFP) was investigated in two Pseudozyma species and compared with other secretion signal sequences, from S. cerevisiae and Pseudozyma spp. The results indicate that various secretion signal sequences were functional and that the P34 signal peptide was the most effective secretion signal sequence in both P. flocculosa and P. antarctica. The cells correctly processed the secretion signal sequences, including P34 signal peptide, and mature GFP was recovered from the culture medium. This is the first report of functional secretion signal sequences in P. flocculosa. These sequences can be used to test the secretion of other recombinant proteins and for studying the secretion pathway in P. flocculosa and P. antarctica.