Effects of Nitrogen Addition on Plant Properties and Microbiomes Under High Phosphorus Addition Level in the Alpine Steppe

• Published in: Frontiers in plant science Vol. 13; p. 894365
• Main Authors: Dong, Junfu, Cui, Xiaoyong, Niu, Haishan, Zhang, Jing, Zhu, Chuanlu, Li, Linfeng, Pang, Zhe, Wang, Shiping
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 20.06.2022
• Subjects: nitrogen application; nutrient uptake; Plant Science; plant–microbe interaction; the core species; the Qinghai-Tibetan plateau
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2022.894365

Nitrogen (N) addition can increase the vegetative growth, improve the plant production, and restore the degraded terrestrial ecosystems. But, it simultaneously aggravates the soil phosphorus (P) limitation for plant growth, thus affecting its positive effects on ecosystems. However, how plants and soil microorganisms will change under conditions of high P content in soil is still unknown. In this study, we explored the effects of three levels of N addition (0, 7.5, and 15 g.N.m –2 .year –1 ) on plants and microorganisms at the high P addition level (13.09 g.P.m –2 .year –1 ) in the alpine steppe. We found that the soil microbial community composition had no significant difference between different N addition levels, and the soil AN and AP had a significant effect on the phospholipid fatty acid (PLFA) composition. The abundance of the core PLFAs (i.e., 16:1ω7c, 16:0, a17:1, i17:0, 18:1ω9c, and 18:1ω7c) also remained unchanged after N addition, and microbes at individual, population, and community levels were all correlated with SOM, AK, AN, and pH. Conversely, plant biomass and nutrient content showed linear trends with increasing N addition, especially the dominant functional groups. Specifically, the biomass and plant tissue N content of Gramineae , and the total N content of aboveground biomass were all improved by N addition. They were correlated with soil ammonium and AP. The structural equation modeling (SEM) demonstrated that N addition had a direct negative effect on soil microbial biomass, but an indirect positive effect on aboveground biomass via soil ammonium. These findings clarify the importance of N-amendment in regulating plants and microorganisms under high P conditions and provide a better understanding of the N-added effects in the alpine steppe.