Genetic regulation of cervical antiinflammatory cytokine concentrations during pregnancy

• Published in: American journal of obstetrics and gynecology Vol. 199; no. 2; pp. 163.e1 - 163.e11
• Main Authors: Simhan, Hyagriv N., MD, MSCR, Ryckman, Kelli K., BS, Williams, Scott M., PhD, Krohn, Marijane A., PhD
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Mosby, Inc 01.08.2008; Elsevier
• Subjects: Adolescent; Adult; Analysis of Variance; antiinflammatory cytokine; Biological and medical sciences; Bones, joints and connective tissue. Antiinflammatory agents; cervix; Cervix Uteri - immunology; Cervix Uteri - metabolism; Diseases of mother, fetus and pregnancy; Female; gene; Gene Frequency; Genotype; Gynecology. Andrology. Obstetrics; Humans; interleukin-10; Interleukin-10 - metabolism; Interleukin-10 Receptor alpha Subunit - metabolism; Interleukin-10 Receptor beta Subunit - metabolism; Interleukin-13 - metabolism; Interleukin-4 - metabolism; Medical sciences; Obstetrics and Gynecology; Pharmacology. Drug treatments; Polymorphism, Single Nucleotide; Pregnancy; Pregnancy Trimester, First - genetics; Pregnancy Trimester, First - immunology; Pregnancy Trimester, First - physiology; Pregnancy. Fetus. Placenta; Premature Birth - genetics; Prospective Studies; Smoking; Vaginosis, Bacterial - metabolism; antiinflammatory cytokine; cervix; gene; interleukin-10; Regulation(control); Pregnancy disorders; Gynecology; Antiinflammatory agent; Genetics; Uterine cervix; Concentration; Ectopic pregnancy; Obstetrics
• ISSN: 0002-9378; 1097-6868
• DOI: 10.1016/j.ajog.2008.02.033

Objective The purpose of this study was to evaluate the contribution of maternal genotype to the concentration of interleukin (IL)-4, −10, and −13 in the cervix during the first trimester in the context of bacterial vaginosis and cigarette smoking. Study Design In this prospective cohort of 116 pregnant women, we examined 72 single nucleotide polymorphisms (SNPs) in 6 genes: IL-4, IL-4 receptor, IL-10, IL-10 receptor α, IL-10 receptor β, and IL-13. Results Among black women, an IL-10 receptor β SNP (rs6517158) demonstrated significant association with IL-10 concentration ( P = .04), adjusted for bacterial vaginosis. Among white women, there were 4 SNPs in IL-10 receptor α with a highly significant association with IL-10 concentration, adjusted for bacterial vaginosis and smoking. Conclusion Our data demonstrate a significant relation between cervical concentrations of IL-10 and SNPs in the IL-10 receptor α and β genes. The contribution of genotype to IL-10 concentration is significant, even in the consideration of bacterial vaginosis and smoking.