Ethanol modified supercritical fluid extraction and antioxidant activity of cajaninstilbene acid and pinostrobin from pigeonpea [ Cajanus cajan (L.) Millsp.] leaves
Supercritical fluid carbon dioxide (SF-CO 2) extraction (SFE) of cajaninstilbene acid (CSA) and pinostrobin (PI) from pigeonpea leaves and antioxidant activity were investigated for the first time. SFE gave higher extraction yields than conventional heat-refluxing extraction (HRE) because of the sev...
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| Published in | Food chemistry Vol. 117; no. 1; pp. 152 - 159 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
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01.11.2009
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| Abstract | Supercritical fluid carbon dioxide (SF-CO
2) extraction (SFE) of cajaninstilbene acid (CSA) and pinostrobin (PI) from pigeonpea leaves and antioxidant activity were investigated for the first time. SFE gave higher extraction yields than conventional heat-refluxing extraction (HRE) because of the severe breakage of cell morphology caused by SF-CO
2, which was observed by scanning electron microscopy (SEM). CO
2 flow rate, modifier composition and liquid to solid ratio were firstly optimised, 12
kg/h, and 80% EtOH with a liquid to solid ratio of 10
ml/g raw material exhibited better extraction performance. Then a central composite design (CCD) combined with response surface methodology was used to study the effects of extraction pressure, temperature and time on the extraction yields of CSA and PI, the optimal parameters were 30
MPa, 60
°C and 2
h. An antioxidant activity investigation showed SFE extracts exhibited better free radical-scavenging activity than HRE extracts with an IC
50 value of 0.2259
mg/ml. |
|---|---|
| AbstractList | Supercritical fluid carbon dioxide (SF-CO
2) extraction (SFE) of cajaninstilbene acid (CSA) and pinostrobin (PI) from pigeonpea leaves and antioxidant activity were investigated for the first time. SFE gave higher extraction yields than conventional heat-refluxing extraction (HRE) because of the severe breakage of cell morphology caused by SF-CO
2, which was observed by scanning electron microscopy (SEM). CO
2 flow rate, modifier composition and liquid to solid ratio were firstly optimised, 12
kg/h, and 80% EtOH with a liquid to solid ratio of 10
ml/g raw material exhibited better extraction performance. Then a central composite design (CCD) combined with response surface methodology was used to study the effects of extraction pressure, temperature and time on the extraction yields of CSA and PI, the optimal parameters were 30
MPa, 60
°C and 2
h. An antioxidant activity investigation showed SFE extracts exhibited better free radical-scavenging activity than HRE extracts with an IC
50 value of 0.2259
mg/ml. Supercritical fluid carbon dioxide (SF-CO₂) extraction (SFE) of cajaninstilbene acid (CSA) and pinostrobin (PI) from pigeonpea leaves and antioxidant activity were investigated for the first time. SFE gave higher extraction yields than conventional heat-refluxing extraction (HRE) because of the severe breakage of cell morphology caused by SF-CO₂, which was observed by scanning electron microscopy (SEM). CO₂ flow rate, modifier composition and liquid to solid ratio were firstly optimised, 12kg/h, and 80% EtOH with a liquid to solid ratio of 10ml/g raw material exhibited better extraction performance. Then a central composite design (CCD) combined with response surface methodology was used to study the effects of extraction pressure, temperature and time on the extraction yields of CSA and PI, the optimal parameters were 30MPa, 60°C and 2h. An antioxidant activity investigation showed SFE extracts exhibited better free radical-scavenging activity than HRE extracts with an IC₅₀ value of 0.2259mg/ml. |
| Author | Fu, Yu-Jie Wang, Wei Yang, Mei Liu, Wei Zu, Yuan-Gang Hua, Xin Kong, Yu |
| Author_xml | – sequence: 1 givenname: Yu surname: Kong fullname: Kong, Yu – sequence: 2 givenname: Yu-Jie surname: Fu fullname: Fu, Yu-Jie email: yujie_fu2002@yahoo.com – sequence: 3 givenname: Yuan-Gang surname: Zu fullname: Zu, Yuan-Gang email: zygorl@vip.hl.cn – sequence: 4 givenname: Wei surname: Liu fullname: Liu, Wei – sequence: 5 givenname: Wei surname: Wang fullname: Wang, Wei – sequence: 6 givenname: Xin surname: Hua fullname: Hua, Xin – sequence: 7 givenname: Mei surname: Yang fullname: Yang, Mei |
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| Keywords | Cajaninstilbene acid Pinostrobin Supercritical fluid carbon dioxide extraction Pigeonpea leaves Antioxidant activity Plant part Ethanol Carbon dioxide Plant leaf Antioxidant Supercritical fluid extraction |
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2) extraction (SFE) of cajaninstilbene acid (CSA) and pinostrobin (PI) from pigeonpea leaves and antioxidant activity... Supercritical fluid carbon dioxide (SF-CO₂) extraction (SFE) of cajaninstilbene acid (CSA) and pinostrobin (PI) from pigeonpea leaves and antioxidant activity... |
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| SubjectTerms | Antioxidant activity Biological and medical sciences Cajaninstilbene acid Cajanus cajan cell structures chemical constituents of plants flavonoids Food industries Fundamental and applied biological sciences. Psychology leaves Pigeonpea leaves Pinostrobin plant extracts pressure stilbenes Supercritical fluid carbon dioxide extraction supercritical fluid extraction temperature yields |
| Title | Ethanol modified supercritical fluid extraction and antioxidant activity of cajaninstilbene acid and pinostrobin from pigeonpea [ Cajanus cajan (L.) Millsp.] leaves |
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