Ethanol modified supercritical fluid extraction and antioxidant activity of cajaninstilbene acid and pinostrobin from pigeonpea [ Cajanus cajan (L.) Millsp.] leaves

• Published in: Food chemistry Vol. 117; no. 1; pp. 152 - 159
• Main Authors: Kong, Yu, Fu, Yu-Jie, Zu, Yuan-Gang, Liu, Wei, Wang, Wei, Hua, Xin, Yang, Mei
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2009; Elsevier
• Subjects: Antioxidant activity; Biological and medical sciences; Cajaninstilbene acid; Cajanus cajan; cell structures; chemical constituents of plants; flavonoids; Food industries; Fundamental and applied biological sciences. Psychology; leaves; Pigeonpea leaves; Pinostrobin; plant extracts; pressure; stilbenes; Supercritical fluid carbon dioxide extraction; supercritical fluid extraction; temperature; yields; Cajaninstilbene acid; Pinostrobin; Supercritical fluid carbon dioxide extraction; Pigeonpea leaves; Antioxidant activity; Plant part; Ethanol; Carbon dioxide; Plant leaf; Antioxidant; Supercritical fluid extraction
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2009.03.091

Supercritical fluid carbon dioxide (SF-CO 2) extraction (SFE) of cajaninstilbene acid (CSA) and pinostrobin (PI) from pigeonpea leaves and antioxidant activity were investigated for the first time. SFE gave higher extraction yields than conventional heat-refluxing extraction (HRE) because of the severe breakage of cell morphology caused by SF-CO 2, which was observed by scanning electron microscopy (SEM). CO 2 flow rate, modifier composition and liquid to solid ratio were firstly optimised, 12 kg/h, and 80% EtOH with a liquid to solid ratio of 10 ml/g raw material exhibited better extraction performance. Then a central composite design (CCD) combined with response surface methodology was used to study the effects of extraction pressure, temperature and time on the extraction yields of CSA and PI, the optimal parameters were 30 MPa, 60 °C and 2 h. An antioxidant activity investigation showed SFE extracts exhibited better free radical-scavenging activity than HRE extracts with an IC 50 value of 0.2259 mg/ml.