Genetic Diversity of Campylobacter jejuni Isolated From Avian and Human Sources in Egypt

Campylobacter jejuni ( C. jejuni ) are able to colonise and infect domestic poultry and also pose a risk for humans. The aim of this study was to determine the extent of genotypic diversity among C. jejuni isolates recovered from avian and human sources in Egypt. Furthermore, the short variable regi...

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Published inFrontiers in microbiology Vol. 10; p. 2353
Main Authors Abd El-Hamid, Marwa I., Abd El-Aziz, Norhan K., Samir, Mohamed, El-Naenaeey, El-sayed Y., Abo Remela, Etab M., Mosbah, Rasha A., Bendary, Mahmoud M.
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 18.10.2019
Subjects
alleles
C. jejuni
flaA typing
humans
Microbiology
poultry
virulence
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Summary:Campylobacter jejuni ( C. jejuni ) are able to colonise and infect domestic poultry and also pose a risk for humans. The aim of this study was to determine the extent of genotypic diversity among C. jejuni isolates recovered from avian and human sources in Egypt. Furthermore, the short variable region (SVR) of flagellin A ( flaA ) gene was analysed for the presence of allelic variants. Our results showed that C. jejuni isolates differ in their capacity to harbour each of the virulence genes alone or when present in various combinations. The flaA gene was detected in all C. jejuni strains and none of the strains had all the studied virulence genes together. When considering C. jejuni strains from the investigated sources, the cdtC gene was the most similar, while the cdtB and iam genes were the most dissimilar. We could identify 13 novel alleles in the analysed strains. The analyses of virulence gene patterns, flaA gene sequences and allelic variants showed that C. jejuni strains from different sources overlapped largely suggesting potential involvement of poultry in transmitting C. jejuni to humans. We also found that the strains isolated from the same host were highly heterogeneous, with chicken strains exhibiting the highest diversity. Moreover, the human strains were clustered closer to chicken ones than to those from pigeon. The results of this study should be taken into consideration when assessing the epidemiology and risk potential of Egyptian C. jejuni not only in poultry, but also in humans.
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Edited by: Stuart A. Thompson, Augusta University, United States
Reviewed by: Mohamed K. Fakhr, The University of Tulsa, United States; Heriberto Fernandez, Austral University of Chile, Chile
This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology
These authors have contributed equally to this work as first authors
ORCID: Marwa I. Abd El-Hamid orcid.org/0000-0002-1560-6158; Norhan K. Abd El-Aziz orcid.org/0000-0001-8309-9058; Mohamed Samir orcid.org/0000-0002-1166-0480; Mahmoud M. Bendary orcid.org/0000-0002-1788-0038
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2019.02353