Oxidative Stress Via Hydrogen Peroxide Affects Proopiomelanocortin Peptides Directly in the Epidermis of Patients with Vitiligo

• Published in: Journal of investigative dermatology Vol. 127; no. 2; pp. 411 - 420
• Main Authors: Spencer, Jennifer D., Gibbons, Nicholas C.J., Rokos, Hartmut, Peters, Eva M.J., Wood, John M., Schallreuter, Karin U.
• Format: Journal Article
• Language: English
• Published: Danvers, MA Elsevier Inc 01.02.2007; Nature Publishing; Elsevier Limited
• Subjects: Adrenocorticotropic Hormone - chemistry; Adrenocorticotropic Hormone - drug effects; alpha-MSH - chemistry; alpha-MSH - drug effects; alpha-MSH - metabolism; beta-Endorphin - chemistry; beta-Endorphin - drug effects; beta-Endorphin - metabolism; Biological and medical sciences; Biopterins - analogs & derivatives; Biopterins - metabolism; Catalase - pharmacology; Cells, Cultured; Computer Simulation; Dermatology; Epidermis - drug effects; Epidermis - metabolism; Fourier Analysis; Humans; Hydrogen Peroxide - antagonists & inhibitors; Hydrogen Peroxide - metabolism; Hydrogen Peroxide - pharmacology; Medical sciences; Melanins - biosynthesis; Models, Biological; Oxidation-Reduction; Oxidative Stress; Peptide Fragments - metabolism; Pigmentary diseases of the skin; Pro-Opiomelanocortin - metabolism; Skin Pigmentation; Spectrum Analysis, Raman; Vitiligo - metabolism; Vitiligo - physiopathology; Human; Oxidative stress; Skin disease; Vitiligo; Pigmentation disorder; Hydrogen peroxide; Peptides; Dermatology; Proopiocortin; Hydrogenperoxides; Epidermis
• ISSN: 0022-202X; 1523-1747
• DOI: 10.1038/sj.jid.5700538

The human skin holds the capacity for autocrine processing of the proopiomelanocortin (POMC)-derived peptides. Recent data demonstrated the presence and functionality of ACTH, α- and β-melanocyte-stimulating hormone (MSH), and β-endorphin in the regulation of skin pigmentation, and a role has been put forward for α-MSH as an effective antioxidant. In patients with vitiligo, decreased epidermal POMC processing and low α-MSH levels were documented previously. These patients accumulate hydrogen peroxide (H2O2) in the 10−3M range in their epidermis. Therefore, we examined the involvement of H2O2 on POMC-derived peptides as possible targets for oxidation by this reactive oxygen species. To address this, we employed immunofluorescence labelling, dot blot analysis, Fourier transform Raman spectroscopy, functionality studies, and computer simulation of the peptide structures. We demonstrate H2O2-mediated oxidation of epidermal ACTH, α-MSH, and β-endorphin in vitiligo owing to oxidation of methionine residues in the sequences of these peptides. Moreover, we show that oxidized β-endorphin loses its function in the promotion of pigmentation in melanocytes. These changes are reversible upon the reduction of H2O2 levels by a pseudocatalase PC-KUS. Moreover, oxidation of α-MSH can be prevented by the formation of a 1:1 complex with the abundant cofactor (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin. Thus, using vitiligo, we demonstrate that H2O2 can affect pigmentation via epidermal POMC peptide redox homeostasis.