Knockdown of PagSAP11 Confers Drought Resistance and Promotes Lateral Shoot Growth in Hybrid Poplar (Populus alba × Populus tremula var. glandulosa)
Plants have evolved defense mechanisms to overcome unfavorable climatic conditions. The growth and development of plants are regulated in response to environmental stress. In this study, we investigated the molecular and physiological characteristics of a novel gene PagSAP11 in hybrid poplar ( Popul...
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Published in | Frontiers in plant science Vol. 13; p. 925744 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
24.06.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Plants have evolved defense mechanisms to overcome unfavorable climatic conditions. The growth and development of plants are regulated in response to environmental stress. In this study, we investigated the molecular and physiological characteristics of a novel gene
PagSAP11
in hybrid poplar (
Populus alba
×
Populus tremula
var.
glandulosa
) under drought stress.
PagSAP11,
a stress-associated protein (SAP) family gene, encodes a putative protein containing an A20 and AN1 zinc-finger domain at its N- and C-termini, respectively. Knockdown of
PagSAP11
transgenic poplars (
SAP11
-Ri) enhanced their tolerance to drought stress compared with wild type plants. Moreover, the RNAi lines showed increased branching of lateral shoots that led to a gain in fresh weight, even when grown in the living modified organism (LMO) field. In
SAP11
-Ri transgenic plants, the expression levels of genes involved in axillary bud outgrowth and cell proliferation such as
DML10
,
CYP707A
and
RAX
were increased while the
DRM
gene which involved in bud dormancy was down-regulated. Taken together, these results indicate that
PagSAP11
represents a promising candidate gene for engineering trees with improved stress tolerance and growth during unfavorable conditions. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Ahmed H. El-Sappah, Zagazig University, Egypt; Wenjing Yao, Nanjing Forestry University, China Edited by: Longxing Hu, Hunan Agricultural University, China This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2022.925744 |