Clonal serotype 1c multidrug-resistant Shigella flexneri detected in multiple institutions by sentinel-site sequencing
Shigella flexneri is a major diarrhoeal pathogen, and the emergence of multidrug-resistant S. flexneri is of public health concern. We report the detection of a clonal cluster of multidrug-resistant serotype 1c (7a) S. flexneri in Singapore in April 2022. Long-read whole-genome sequence analysis fou...
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Published in | Frontiers in medicine Vol. 9; p. 964640 |
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Main Authors | , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
01.08.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Shigella flexneri
is a major diarrhoeal pathogen, and the emergence of multidrug-resistant
S. flexneri
is of public health concern. We report the detection of a clonal cluster of multidrug-resistant serotype 1c (7a)
S. flexneri
in Singapore in April 2022. Long-read whole-genome sequence analysis found five
S. flexneri
isolates to be clonal and harboring the extended-spectrum β-lactamases
bla
CTX−M−15
and
bla
TEM−1
. The isolates were phenotypically resistant to ceftriaxone and had intermediate susceptibility to ciprofloxacin. The
S. flexneri
clonal cluster was first detected in a tertiary hospital diagnostic laboratory (sentinel-site), to which the
S. flexneri
isolates were sent from other hospitals for routine serogrouping. Long-read whole-genome sequence analysis was performed in the sentinel-site near real-time in view of the unusually high number of
S. flexneri
isolates received within a short time frame. This study demonstrates that near real-time sentinel-site sequence-based surveillance of convenience samples can detect possible clonal outbreak clusters and may provide alerts useful for public health mitigations at the earliest possible opportunity. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Morteza Saki, Ahvaz Jundishapur University of Medical Sciences, Iran; F.-X. Campbell-Valois, University of Ottawa, Canada This article was submitted to Infectious Diseases - Surveillance, Prevention and Treatment, a section of the journal Frontiers in Medicine Edited by: Milad Badri, Qazvin University of Medical Sciences, Iran |
ISSN: | 2296-858X 2296-858X |
DOI: | 10.3389/fmed.2022.964640 |