Assignment of the Human B-Cell-Derived (BCD1) Proto-oncogene to 10p14–p15
The B-cell-derived (BCD1) gene, recently cloned from the peripheral blood lymphocytes of one B-cell chronic lymphocytic leukemia (B-CLL) patient due to its capacity to transform NIH 3T3 cells, represents a novel proto-oncogene. The BCD1 gene is limited in expression to two tissues, CD19 super(+) B-c...
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Published in | Genomics (San Diego, Calif.) Vol. 43; no. 3; pp. 395 - 397 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
San Diego, CA
Elsevier Inc
01.08.1997
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | The B-cell-derived (BCD1) gene, recently cloned from the peripheral blood lymphocytes of one B-cell chronic lymphocytic leukemia (B-CLL) patient due to its capacity to transform NIH 3T3 cells, represents a novel proto-oncogene. The BCD1 gene is limited in expression to two tissues, CD19 super(+) B-cells or testis of normal individuals. Malignant B-cells from 50% of B-CLL patients show no detectable BCD1 gene transcripts. However, when malignant B-cells are stimulated to undergo terminal differentiation into plasma cells, BCD1 gene expression was induced, suggesting an association with B-cell maturation. The BCD1 gene product shows high homology with two human transcription factors BTEB2 (basic transcriptional element binding protein) and SP1, which are GC-box-binding proteins. In this study we used a cDNA containing the BCD1 gene as a probe for Southern blot analysis and fluorescence in situ hybridization (FISH) and have mapped the BCD1 gene to human chromosome 10 band p14-p15. DNAs from a variety of other species also contained sequences homologous to BCD1, showing that it is highly conserved in evolution, which suggests that it may play an important role in regulating B-cell growth and differentiation. The human BCD1 locus lies within a region of 10p that shows frequent translocations in lymphoid malignancies. The chromosomal assignment of the BCD1 gene was determined by Southern blot analysis using a somatic cell hybrid panel of 20 human/hamster and human/mouse cell lines (BIOS) that contained a single chromosome or several different human chromosomes in one or more hybrid cell lines. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0888-7543 1089-8646 |
DOI: | 10.1006/geno.1997.4824 |