Assignment of the Human B-Cell-Derived (BCD1) Proto-oncogene to 10p14–p15

• Published in: Genomics (San Diego, Calif.) Vol. 43; no. 3; pp. 395 - 397
• Main Authors: Rouby, Soumaya El, Rao, P.H., Newcomb, Elizabeth W.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.08.1997; Elsevier
• Subjects: Animals; Biological and medical sciences; Blotting, Southern; Chromosome Mapping; Chromosomes, Human, Pair 10 - genetics; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Human; Humans; Hybrid Cells - metabolism; In Situ Hybridization, Fluorescence; Kruppel-Like Factor 6; Kruppel-Like Transcription Factors; Mice; Proto-Oncogene Proteins - genetics; Trans-Activators; Genetic mapping; Human; B-Lymphocyte; Chromosome C10; Transcription factor; C-Onc gene
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1997.4824

The B-cell-derived (BCD1) gene, recently cloned from the peripheral blood lymphocytes of one B-cell chronic lymphocytic leukemia (B-CLL) patient due to its capacity to transform NIH 3T3 cells, represents a novel proto-oncogene. The BCD1 gene is limited in expression to two tissues, CD19 super(+) B-cells or testis of normal individuals. Malignant B-cells from 50% of B-CLL patients show no detectable BCD1 gene transcripts. However, when malignant B-cells are stimulated to undergo terminal differentiation into plasma cells, BCD1 gene expression was induced, suggesting an association with B-cell maturation. The BCD1 gene product shows high homology with two human transcription factors BTEB2 (basic transcriptional element binding protein) and SP1, which are GC-box-binding proteins. In this study we used a cDNA containing the BCD1 gene as a probe for Southern blot analysis and fluorescence in situ hybridization (FISH) and have mapped the BCD1 gene to human chromosome 10 band p14-p15. DNAs from a variety of other species also contained sequences homologous to BCD1, showing that it is highly conserved in evolution, which suggests that it may play an important role in regulating B-cell growth and differentiation. The human BCD1 locus lies within a region of 10p that shows frequent translocations in lymphoid malignancies. The chromosomal assignment of the BCD1 gene was determined by Southern blot analysis using a somatic cell hybrid panel of 20 human/hamster and human/mouse cell lines (BIOS) that contained a single chromosome or several different human chromosomes in one or more hybrid cell lines.