Nanostructured zinc oxide platform for mycotoxin detection

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Vol. 77; no. 2; pp. 75 - 81
• Main Authors: Ansari, Anees A., Kaushik, Ajeet, Solanki, Pratima R., Malhotra, B.D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2010
• Subjects: Electric Impedance; Electrochemistry; Enzymes, Immobilized - chemistry; Glass - chemistry; Immunoglobulin G - immunology; Impedimetric immunosensor; Microelectrodes; Microscopy, Electron, Scanning; Mycotoxin; Mycotoxins - analysis; Nanostructures - chemistry; Ochratoxin-A; Ochratoxins - analysis; Particle Size; Reproducibility of Results; Sensitivity and Specificity; Serum Albumin, Bovine - chemistry; Sol–gel; Spectroscopy, Fourier Transform Infrared; Tin Compounds - chemistry; X-Ray Diffraction; Zinc oxide; Zinc Oxide - chemistry; Zinc oxide; Impedimetric immunosensor; Sol–gel; Ochratoxin-A; Mycotoxin
• ISSN: 1567-5394; 1878-562X
• DOI: 10.1016/j.bioelechem.2009.06.014

Nanostructured zinc oxide (Nano-ZnO) film has been deposited onto indium–tin–oxide (ITO) glass plate for co-immobilization of rabbit-immunoglubin antibodies (r-IgGs) and bovine serum albumin (BSA) for ochratoxin-A (OTA) detection. The results of X-ray diffraction (XRD) studies reveal the formation of Nano-ZnO with average particle size as ~5.0 nm. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) techniques have been used to characterize Nano-ZnO/ITO electrode and BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode. Electrochemical impedimetric response of BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode obtained as a function of OTA concentration exhibits linearity as 0.006–0.01 nM/dm 3, detection limit of 0.006 nM/dm 3, response time as 25 s and sensitivity of 189 Ω/nM/dm 3cm − 2 with a regression coefficient of 0.997.