Astrocytic expression of GFAP and serum levels of IL-1β and TNF-α in rats treated with different pain relievers

• Published in: Brazilian Journal of Pharmaceutical Sciences Vol. 52; no. 4; pp. 623 - 633
• Main Authors: Amaral, Gisele Ferreira, Dossa, Pietro Domingues, Viebig, Lígia Bocamino, Konno, Fabiana Toshie Camargo, Consoli, Amanda, Martins, Maria de Fátima Monteiro, Viani, Flávio Cesar, Bondan, Eduardo Fernandes
• Format: Journal Article
• Language: English
• Published: Sao Paulo Universidade de Sao Paulo Faculdade de Ciencias 01.10.2016; Universidade de São Paulo, Faculdade de Ciências Farmacêuticas; Universidade de São Paulo
• Subjects: Analgesics/tests/treatment; Antibodies; Astrocytes/pain regulation; Cytokines; Drug dosages; Glial Fibrillary Acidic Protein/study; Laboratory animals; Narcotics; Neuropathic pain/treatment; Ostomy; Pain; PHARMACOLOGY & PHARMACY; Tumor necrosis factor-TNF; Variance analysis; Glial Fibrillary Acidic Protein/study; Cytokines; Analgesics/tests/treatment; Astrocytes/pain regulation; Neuropathic pain/treatment
• ISSN: 1984-8250; 2175-9790; 1984-8250; 2175-9790
• DOI: 10.1590/s1984-82502016000400006

Pro-inflammatory cytokines and glial cells, especially microglial cells, have been implicated in persistent pain sensitization. Less is known about the role of astrocytes in pain regulation. This study aimed to observe the expression of the astrocytic biomarker glial fibrillary acidic protein (GFAP) and the serum levels of interleukin 1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) after short-term administration of central pain relievers in rats not submitted to noxious stimuli. Male Wistar rats were divided into five groups, receiving for nine days- (1) amitriptyline (Amt-10 mg/kg/day, by gavage); (2) gabapentin (Gb-60 mg/kg/day, by gavage; (3) methadone (Me-4.5 mg/kg/day, intraperitoneal route [IP]); (4) morphine (Mo-10 mg/kg/day, IP); or (5) 0.9% saline solution, IP. Brain samples were collected for immunohistochemical study of GFAP expression in the mesencephalon and nucleus accumbens (NAc). The area of GFAP-positive cells was calculated using MetaMorph software and serum levels of IL-1β and TNF-α were measured by enzyme-linked immunosorbent assay. Serum TNF-α levels were decreased in the groups treated with Mo, Me and Gb, but not in the Amt-treated group. IL-1β decreased only in rats treated with Me. The astrocytic expression of GFAP was decreased in the brainstem with all drugs, while it was increased in the NAc with Amt, Me and Mo.