Preparation and Characterization of an Optimized Meniscal Extracellular Matrix Scaffold for Meniscus Transplantation
Many studies have sought to construct a substitute to partially replace irreparably damaged meniscus. Only the meniscus allograft has been used in clinical practice as a useful substitute, and there are concerns about its longevity and inherent limitations, including availability of donor tissue and...
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Published in | Frontiers in bioengineering and biotechnology Vol. 8; p. 779 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
09.07.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Many studies have sought to construct a substitute to partially replace irreparably damaged meniscus. Only the meniscus allograft has been used in clinical practice as a useful substitute, and there are concerns about its longevity and inherent limitations, including availability of donor tissue and possibility of disease transmission. To overcome these limitations, we developed an acellular xenograft from whole porcine meniscus. Samples were treated with 2% Triton X-100 for 10 days and 2% sodium dodecyl sulfate for 6 days. The DNA content of extracellular matrix (ECM) scaffolds was significantly decreased compared with that of normal porcine menisci (
p
< 0.001). Histological analysis confirmed the maintenance of ECM integrity and anisotropic architecture in the absence of nuclei. Biochemical and biomechanical assays of the scaffolds indicated the preservation of collagen (
p
= 0.806), glycosaminoglycan (
p
= 0.188), and biomechanical properties (elastic modulus and transition stress). The scaffolds possessed good biocompatibility and supported bone marrow mesenchymal stem cells (BMSCs) proliferation for 2 weeks
in vitro
, with excellent region-specific recellularization
in vivo
. The novel scaffold has potential value for application in recellularization and transplantation strategies. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Tracy Tong Li Yu, NxR Biotechnologies GmbH, Switzerland Reviewed by: Adetola B. Adesida, University of Alberta, Canada; Caroline Curtin, Royal College of Surgeons in Ireland, Ireland These authors have contributed equally to this work This article was submitted to Tissue Engineering and Regenerative Medicine, a section of the journal Frontiers in Bioengineering and Biotechnology |
ISSN: | 2296-4185 2296-4185 |
DOI: | 10.3389/fbioe.2020.00779 |