Effects of BrMYC2/3/4 on Plant Development, Glucosinolate Metabolism, and Sclerotinia sclerotiorum Resistance in Transgenic Arabidopsis thaliana
MYC2/3/4, known as a basic helix–loop–helix (bHLH) transcription factor, directly activate the genes involved in diverse plant development and secondary metabolites biosynthesis. In this study, we identified and cloned five MYC paralogs ( BrMYC2 / 3-1 / 3-2 / 4-1 / 4-2 ) from Chinese cabbage ( Brass...
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Published in | Frontiers in plant science Vol. 12; p. 707054 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
03.09.2021
|
Subjects | |
Online Access | Get full text |
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Summary: | MYC2/3/4, known as a basic helix–loop–helix (bHLH) transcription factor, directly activate the genes involved in diverse plant development and secondary metabolites biosynthesis. In this study, we identified and cloned five
MYC
paralogs (
BrMYC2
/
3-1
/
3-2
/
4-1
/
4-2
) from Chinese cabbage (
Brassica rapa
ssp.
pekinensis
).
In-silico
analyses for the physicochemical properties suggested that BrMYC2/3-1/3-2/4-2/4-3 are unstable hydrophobic and acidic proteins, while BrMYC4-1 is an unstable hydrophobic and basic protein. BrMYC2/3/4 belong to the bHLH superfamily and are closely related to AthMYC2/3/4 orthologs that mediate the regulation of various secondary metabolites. It was demonstrated that BrMYC2/3/4-GFP fusion protein localized in the nucleus and expression levels of five
BrMYC2/3/4
homologous genes all elevated relative to control (Ctrl). When expressed in
Arabidopsis
under the control of 35S promoter, each of the
BrMYC2/3-1/3-2/4-1/4-2
transgenes differentially influenced root and shoot elongation, vegetative phase change, flowering time, plant height and tiller number after flowering, and seed production. Despite the variation of phenotypes between the transgenic lines, all the lines except for
BrMYC4-2
exhibited shorter seed length, less seed weight, higher accumulation of glucosinolates (GSs), and resistance to
Sclerotinia sclerotiorum
than Ctrl. Notably,
BrMYC2
overexpression (OE) line significantly reduced the lengths of root and hypocotyl, seed length, and weight, along with faster bolting time and strikingly higher accumulation of total GSs. Accumulation of GSs at the highest levels in the
BrMYC2
OE
line conferred the highest resistance to
S. sclerotiorum
. Unlike
BrMYC3
OE
and
BrMYC4
OE
,
BrMYC2
OE
stimulated the growth of plant height after fluorescence. The results of this study point to the
BrMYC2
overexpression that may provide a beneficial effect on plant growth and development
via
plant resistance to the fungal pathogen. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science These authors have contributed equally to this work Edited by: Stephan Pollmann, National Institute of Agricultural and Food Research and Technology, Spain Reviewed by: Branka Salopek Sondi, Rudjer Boskovic Institute, Croatia; Arvind H. Hirani, Kemin Industries, Inc, United States |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2021.707054 |