Effects of Adenoviral Gene Transduction on the Stemness of Human Bone Marrow Mesenchymal Stem Cells

• Published in: Molecules and cells Vol. 40; no. 8; pp. 598 - 605
• Main Authors: Marasini, Subash, Chang, Da-Young, Jung, Jin-Hwa, Lee, Su-Jung, Cha, Hye Lim, Suh-Kim, Haeyoung, Kim, Sung-Soo
• Format: Journal Article
• Language: English
• Published: United States Korean Society for Molecular and Cellular Biology 01.08.2017; 한국분자세포생물학회
• Subjects: Adenoviridae - metabolism; Adolescent; Antigens, Surface - metabolism; Biomarkers - metabolism; Bone Marrow Cells - cytology; Cell Differentiation; Cell Lineage; Cell Proliferation; Child; Genetic Vectors - metabolism; Green Fluorescent Proteins - metabolism; Humans; Kinetics; Mesenchymal Stem Cells - metabolism; Mesoderm - cytology; Transduction, Genetic; Transgenes; 생물학; mesenchymal stem cells; stemness; adenoviral vector
• ISSN: 1016-8478; 0219-1032
• DOI: 10.14348/molcells.2017.0095

Human mesenchymal stem cells (MSCs) are currently being evaluated as a cell-based therapy for tissue injury and degenerative diseases. Recently, several methods have been suggested to further enhance the therapeutic functions of MSCs, including genetic modifications with tissue- and/or disease-specific genes. The objective of this study was to examine the efficiency and stability of transduction using an adenoviral vector in human MSCs. Additionally, we aimed to assess the effects of transduction on the proliferation and multipotency of MSCs. The results indicate that MSCs can be transduced by adenoviruses , but high viral titers are necessary to achieve high efficiency. In addition, transduction at a higher multiplicity of infection (MOI) was associated with attenuated proliferation and senescence-like morphology. Furthermore, transduced MSCs showed a diminished capacity for adipogenic differentiation while retaining their potential to differentiate into osteocytes and chondrocytes. This work could contribute significantly to clinical trials of MSCs modified with therapeutic genes.