Transcription Factor GmWRKY46 Enhanced Phosphate Starvation Tolerance and Root Development in Transgenic Plants
Phosphorus (P) is one of the essential macronutrients, whose deficiency limits the growth and development of plants. In this study, we investigated the possible role of GmWRKY46 in the phosphate (Pi) starvation stress tolerance of soybean. GmWRKY46 belonged to the group III subfamily of the WRKY tra...
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Published in | Frontiers in plant science Vol. 12; p. 700651 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
14.09.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Phosphorus (P) is one of the essential macronutrients, whose deficiency limits the growth and development of plants. In this study, we investigated the possible role of
GmWRKY46
in the phosphate (Pi) starvation stress tolerance of soybean. GmWRKY46 belonged to the group III subfamily of the WRKY transcription factor family, which was localized in the nucleus and had transcriptional activator activity.
GmWRKY46
could be strongly induced by Pi starvation, especially in soybean roots. Overexpression of
GmWRKY46
significantly enhanced tolerance to Pi starvation and lateral root development in transgenic
Arabidopsis
. RNA-seq analysis showed that overexpression of
GmWRKY46
led to change in many genes related to energy metabolisms, stress responses, and plant hormone signal transduction in transgenic
Arabidopsis
. Among these differential expression genes, we found that overexpression of
AtAED1
alone could enhance the tolerance of transgenic
Arabidopsis
to Pi starvation. Y1H and ChIP-qPCR analyses showed that GmWRKY46 could directly bind to the W-box motif of the
AtAED1
promoter
in vitro
and
in vivo
. Furthermore, results from intact soybean composite plants with
GmWRKY46
overexpression showed that
GmWRKY46
was involved in hairy roots development and subsequently affected plant growth and Pi uptake. These results provide a basis for the molecular genetic breeding of soybean tolerant to Pi starvation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Shisong Ma, University of Science and Technology of China, China; Yuanyuan Zhang, Institute of Botany, Chinese Academy of Sciences, China Edited by: Karam B. Singh, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2021.700651 |