Transcriptome Analysis Reveals the Complex Molecular Mechanisms of Brassica napus–Sclerotinia sclerotiorum Interactions
Sclerotinia stem rot caused by Sclerotinia sclerotiorum is a devastating disease for many important crops worldwide, including Brassica napus . Although numerous studies have been performed on the gene expression changes in B. napus and S. sclerotiorum , knowledge regarding the molecular mechanisms...
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Published in | Frontiers in plant science Vol. 12; p. 716935 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
08.10.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Sclerotinia stem rot caused by
Sclerotinia sclerotiorum
is a devastating disease for many important crops worldwide, including
Brassica napus
. Although numerous studies have been performed on the gene expression changes in
B. napus
and
S. sclerotiorum
, knowledge regarding the molecular mechanisms of
B. napus
–
S. sclerotiorum
interactions is limited. Here, we revealed the changes in the gene expression and related pathways in both
B. napus
and
S. sclerotiorum
during the sclerotinia stem rot (SSR) infection process using transcriptome analyses. In total, 1,986, 2,217, and 16,079 differentially expressed genes (DEGs) were identified in
B. napus
at 6, 24, and 48 h post-inoculation, respectively, whereas 1,511, 1,208, and 2,051 DEGs, respectively, were identified in
S. sclerotiorum
. The gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that most of the hormone-signaling pathways in
B. napus
were enriched, and thus, the hormone contents at four stages were measured. The DEGs and hormone contents revealed that salicylic acid was activated, while the jasmonic acid pathway was repressed at 24 h post-inoculation. Additionally, the expressional patterns of the cell wall-degrading enzyme-encoding genes in
S. sclerotiorum
and the hydrolytic enzymes in
B. napus
were consistent with the SSR infection process. The results contribute to a better understanding of the interactions between
B. napus
and
S. sclerotiorum
and the development of future preventive measures against SSR. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Pilar Soengas, Misión Biológica de Galicia, Consejo Superior de Investigaciones Científicas (CSIC), Spain; Xiao-Li Tan, Jiangsu University, China Edited by: Chunyu Zhang, Huazhong Agricultural University, China These authors have contributed equally to this work This article was submitted to Plant Breeding, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2021.716935 |