Morphological and Immunohistochemical Changes in Muscle Tissue in Association With Mandibular Distraction Osteogenesis

• Published in: Journal of oral and maxillofacial surgery Vol. 65; no. 8; pp. 1517 - 1525
• Main Authors: Sato, Masaru, DDS, Maruoka, Yutaka, DDS, PhD, Kunimori, Kentaro, DDS, Imai, Hideki, DDS, PhD, Kabasawa, Yuji, DDS, PhD, Ichinose, Shizuko, PhD, Harada, Kiyoshi, DDS, PhD, Omura, Ken, DDS, PhD
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.08.2007; Elsevier
• Subjects: Adaptation, Physiological; Animals; Biological and medical sciences; Bone Regeneration - physiology; Dentistry; Immunohistochemistry; Male; Mandible - physiology; Mandible - surgery; Medical sciences; Muscle Fibers, Skeletal - metabolism; Muscle Fibers, Skeletal - physiology; Muscle Fibers, Skeletal - ultrastructure; Neck Muscles - metabolism; Neck Muscles - physiology; Neck Muscles - ultrastructure; Osteogenesis, Distraction; Otorhinolaryngology. Stomatology; Proliferating Cell Nuclear Antigen - metabolism; Rabbits; Regeneration - physiology; Stress, Mechanical; Surgery; Time Factors; Immunohistochemistry; Tissue; Anatomic pathology; Mandible; Association; Treatment; Stomatology; Surgery; Morphological change; Muscle; Distraction osteogenesis
• ISSN: 0278-2391; 1531-5053
• DOI: 10.1016/j.joms.2006.10.041

Purpose Studies of changes in the surrounding soft tissue in association with distraction osteogenesis in the maxillofacial region, where various different kinds of tissue are tightly packed, are rare. In this study, we performed morphological and immunohistochemical investigations of muscle tissue during mandibular distraction osteogenesis. Materials and Methods Japanese white rabbits were divided into 2 groups. In 1 group, the mandibular bone was distracted at a rate of 1 mm/day (DO group), whereas in the other group it was advanced by 10 mm all at once (O group). The cross-section of the anterior belly of the digastric muscle was examined using hematoxylin and eosin, periodic acid-Schiff (PAS), and proliferating cell nuclear antigen (PCNA) staining methods, as well as scanning electronmicroscopy (SEM). Results In the DO group, the cross-section of muscle bundle revealed no remarkable changes. In PAS staining, type II fibers gradually disappeared with distraction, but eventually reappeared after a period of consolidation. In the SEM photographs, striation-like laminated structures were evident in the control and the 3-mm distraction, ambiguous in the 6-mm distraction, and not evident in the 10-mm distraction. In contrast, the structure appeared to be restored in the consolidation for 1 week thereafter. In the PCNA immunostaining, the numbers of PCNA-positive nuclei increased during periods of distraction, but subsequently tended to decrease gradually. Conclusions Although minute injuries were induced in muscle fibers in association with distraction osteogenesis, it is suggested that muscle fibers regenerate starting in the distraction period and thus can adapt to the environment.