Identification and Characterization of Regulatory Pathways Controlling Dormancy Under Lower Temperature in Alfalfa (Medicago sativa L.)
Alfalfa ( Medicago sativa L.), a kind of high-quality perennial legume forage, is widely distributed in the northern regions of China. In recent years, low temperatures have frequently occurred and limited alfalfa productivity and survival in early spring and late fall. However, the underlying molec...
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Published in | Frontiers in plant science Vol. 13; p. 872839 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
02.06.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Alfalfa (
Medicago sativa
L.), a kind of high-quality perennial legume forage, is widely distributed in the northern regions of China. In recent years, low temperatures have frequently occurred and limited alfalfa productivity and survival in early spring and late fall. However, the underlying molecular mechanisms of alfalfa response to cold tolerance are not well-documented. In this study, dormancy and non-dormancy alfalfa standard varieties were characterized under low-temperature stress. Our analysis revealed that plant height of the dormancy genotype was strongly inhibited by low temperature; flavonoids content, and higher expression of flavonoids biosynthesis genes (
chalcone synthase, leucoanthocyanidin dioxygenase
, and
flavonoid 3'-monooxygenase
) may play essential roles in response to low-temperature stress in dormancy genotype alfalfa. Further analyses revealed that receptor-like kinase family genes (such as
cysteine-rich RLK10, lectin protein kinase
, and
S-locus glycoprotein like kinase
), RNA and protein synthesis genes (
RNA polymerases, ribosomal protein
, and
protein phosphatase 2C family protein
), and proteasome degradation pathway genes (such as
F-box family protein, RING/U-box superfamily protein
, and
zinc finger family protein
) also highly upregulated and contributed to cold tolerance phenotype in dormancy genotype alfalfa. This will provide new insights into future studies for cold tolerance in alfalfa and offer new target genes for further functional characterization and genetic improvement of alfalfa. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Bingru Huang, Rutgers, The State University of New Jersey, United States Reviewed by: Longxing Hu, Hunan Agricultural University, China; Zhulong Chan, Huazhong Agricultural University, China This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2022.872839 |