Measurement of cellular excitability by whole cell patch clamp technique

Patch clamp method developed more than 30 years ago is widely used for investigation of cellular excitability manifested as transmembrane ionic current and/or generation of action potentials. This technique could be applied to measurement of ionic currents flowing through individual (single) ion cha...

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Bibliographic Details
Published inPhysiological research Vol. 59 Suppl 1; pp. S1 - S7
Main Authors Karmažínová, M, Lacinová, L
Format Journal Article
LanguageEnglish
Published Czech Republic Institute of Physiology 2010
Subjects
Action Potentials
Animals
Calcium
Calcium Channels, T-Type - metabolism
Calcium Signaling
Cell Membrane - metabolism
Cells
Humans
Ion Channel Gating
Ion Channels - metabolism
Laboratory animals
Membrane Potentials
Models, Biological
Patch-Clamp Techniques
Proteins
R&D
Research & development
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Summary:Patch clamp method developed more than 30 years ago is widely used for investigation of cellular excitability manifested as transmembrane ionic current and/or generation of action potentials. This technique could be applied to measurement of ionic currents flowing through individual (single) ion channels or through the whole assembly of ion channels expressed in the whole cell. Whole cell configuration is more common for measurement of ion currents and the only one enabling measurement of action potentials. This method allows detailed analysis of mechanisms and structural determinants of voltage-dependent gating of ion channels as well as regulation of channel activity by intracellular signaling pathways and pharmacological agents.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
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ObjectType-Review-1
ISSN:0862-8408
1802-9973
DOI:10.33549/physiolres.932000