Extracellular MicroRNAs and Mitochondrial DNA as Potential Biomarkers of Arrhythmogenic Cardiomyopathy

• Published in: Biochemistry (Moscow) Vol. 84; no. 3; pp. 272 - 282
• Main Authors: Khudiakov, A. A., Smolina, N. A., Perepelina, K. I., Malashicheva, A. B., Kostareva, A. A.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.03.2019; Springer; Springer Nature B.V
• Subjects: Arrhythmia; Arrhythmogenic Right Ventricular Dysplasia - diagnosis; Biochemistry; Biological markers; Biomarkers; Biomarkers - analysis; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Cardiomyocytes; Cardiomyopathy; Cells, Cultured; Conditioning; Deoxyribonucleic acid; Differential diagnosis; DNA; DNA, Mitochondrial - analysis; DNA, Mitochondrial - genetics; Genetic aspects; Humans; Identification and classification; Life Sciences; Microbiology; MicroRNA; MicroRNAs; MicroRNAs - analysis; MicroRNAs - genetics; miRNA; Mitochondrial DNA; Myocardial diseases; Nucleic acids; Pluripotency; Stem cell transplantation; Stem cells; Russia; cardiomyocytes; microRNA; induced pluripotent stem cells; arrhythmogenic cardiomyopathy; mitochondrial DNA
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S000629791903009X

Differential diagnosis of arrhythmogenic cardiomyopathy (ACM) during the disease latent phase is a challenging clinical problem that requires identification of early ACM biomarkers. Because extracellular nucleic acids are stable, specific, and can be easily detected, they can be used as reliable biomarkers of various diseases. In this study, we analyzed the levels of extracellular microRNAs and mitochondrial DNA in the conditioned medium collected from cardiomyocytes differentiated from induced pluripotent stem cells of ACM patients and healthy donor. Several microRNAs were expressed differently by the affected and healthy cardiomyocytes; therefore, they could be considered as potential ACM biomarkers.