Adhesion, growth and detachment of cells on modified polystyrene surface

By adsorbing poly(N-isopropylacrylamide) (PNIPAAm) from an aqueous solution onto oxidised polystyrene without the need for grafting the polymer to the surface, we showed here that cells(CHO-K1) adhere and grow well at 37 degrees C and are detached by lowering the temperature to 10 degrees C without...

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Bibliographic Details
Published inCytotechnology (Dordrecht) Vol. 36; no. 1-3; pp. 49 - 53
Main Authors HENDRICK, V, MUNIZ, E, GEUSKENS, G, WERENNE, J
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.07.2001
Springer Nature B.V
Kluwer Academic Publishers
Subjects
Biological and medical sciences
Biotechnology
Cell culture
Fundamental and applied biological sciences. Psychology
Poly(N-isopropylacrylamide)
Polystyrene
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Summary:By adsorbing poly(N-isopropylacrylamide) (PNIPAAm) from an aqueous solution onto oxidised polystyrene without the need for grafting the polymer to the surface, we showed here that cells(CHO-K1) adhere and grow well at 37 degrees C and are detached by lowering the temperature to 10 degrees C without any other deleterious treatment. Both bacterial culture grade polystyrene Petri dishes and polystyrene beads (120 to 250mum diameters) commercially available used in static conditions of growth were tested with similar results. The contact angle of modified Petri dishes with a water droplet increases from 36 to 58 degrees when the temperature is raised from 25 to 37 degrees C indicating change in hydrophilicity of the surface as a function of temperature.
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ISSN:0920-9069
1573-0778
DOI:10.1023/A:1014041003617