Cellulose accessibility and microbial community in solid state anaerobic digestion of rape straw

• Published in: Bioresource technology Vol. 223; pp. 192 - 201
• Main Authors: Tian, Jiang-Hao, Pourcher, Anne-Marie, Bureau, Chrystelle, Peu, Pascal
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2017; Elsevier
• Subjects: Anaerobiosis - physiology; Archaea - metabolism; Bacteroides - metabolism; Biomass; Bioreactors - microbiology; Biota - physiology; Brassica rapa - chemistry; Brassica rapa - metabolism; Cellulase - metabolism; Cellulose - metabolism; Cellulose accessibility; Digestion; Environmental Sciences; Leach bed reactor; Methane - biosynthesis; Methane production kinetics; Microbial community; Pilot Projects; Rape straw; Solid-state anaerobic digestion; Sphingobacterium - metabolism; Water Pollutants, Chemical - chemistry; Water Pollutants, Chemical - metabolism; Rape straw; Solid-state anaerobic digestion; Microbial community; Leach bed reactor; Cellulose accessibility; Methane production kinetics; LEACH BED REACTORS; RAPE STRAWS; BIODEGRADATION; LEACH BED REACTOR; TRANSPORTATION; METHANE PRODUCTION KINETICS; BACTEROIDALES; AGRICULTURE; MICROBIAL COMMUNITY; SOLID-STATE ANAEROBIC DIGESTIONS; RAPE STRAW; CELLULOSE ACCESSIBILITY; MICROORGANISMS; METHANE; ARCHAEA; MICROBIAL COMMUNITIES; SPHINGOBACTERIALES; SOLID-STATE ANAEROBIC DIGESTION; CELLULOSE ACCESSIBILITIES; METHANE PRODUCTION; ANAEROBIC DIGESTION; BACTERIA (MICROORGANISMS); CELLULOSE
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2016.10.009

•Rapeseed straw is a potential feedstock for SSAD.•Improving cellulose accessibility improves the methane yield and production rate.•SSAD of rape straw reached 60% of its BMP in a 40-day pilot-scale test.•Distinct microbial communities were observed in digested rape straw and leachate. Solid state anaerobic digestion (SSAD) with leachate recirculation is an appropriate method for the valorization of agriculture residues. Rape straw is a massively produced residue with considerable biochemical methane potential, but its degradation in SSAD remains poorly understood. A thorough study was conducted to understand the performance of rape straw as feedstock for laboratory solid state anaerobic digesters. We investigated the methane production kinetics of rape straw in relation to cellulose accessibility to cellulase and the microbial community. Improving cellulose accessibility through milling had a positive influence on both the methane production rate and methane yield. The SSAD of rape straw reached 60% of its BMP in a 40-day pilot-scale test. Distinct bacterial communities were observed in digested rape straw and leachate, with Bacteroidales and Sphingobacteriales as the most abundant orders, respectively. Archaeal populations showed no phase preference and increased chronologically.