Thymic Stromal Lmphopoietin Pomotes Macrophage-derived Foam Cell Formation

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 1; pp. 23 - 28
• Main Author: 李大主 王博远 杨保杰 何少林 林静 董江川 吴纯 胡骏
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2014; Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,China
• Subjects: ABCA1; Animals; Antibodies - immunology; Antibodies - pharmacology; ATP Binding Cassette Transporter 1 - genetics; ATP Binding Cassette Transporter 1 - metabolism; Blotting, Western; CD36 Antigens - genetics; CD36 Antigens - metabolism; Cells, Cultured; Cholesterol - metabolism; Cholesterol Esters - metabolism; Cytokines - pharmacology; Dose-Response Relationship, Drug; Foam Cells - cytology; Foam Cells - drug effects; Foam Cells - metabolism; Gene Expression - drug effects; Immunoglobulins - immunology; Immunoglobulins - metabolism; Lipoproteins, LDL - pharmacology; Macrophages - cytology; Macrophages - drug effects; Macrophages - metabolism; Medicine; Medicine & Public Health; Mice; Mice, Inbred C57BL; Receptors, Cytokine - immunology; Receptors, Cytokine - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Scavenger Receptors, Class A - genetics; Scavenger Receptors, Class A - metabolism; Western印迹法; 基质; 巨噬细胞; 氧化低密度脂蛋白; 泡沫细胞; 清道夫受体; 胸腺; atherosclerosis; foam cells; thymic stromal lymphopoietin
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1226-z

The effect of thymic stromal lymphopoietin（TSLP） on macrophage-derived foam cell formation and the underlying mechanism were studied. Macrophages isolated from C57BL/6 mice were co-cultured in vitro with different concentrations of TSLP or TSLPR-antibody in the presence of oxidized low density lipoprotein（ox-LDL）. The effects of TSLP on macrophage-derived foam cell formation were observed by using oil red O staining and intracellular lipid determination. The expression levels of foam cell scavenger receptors（CD36 and SRA） as well as ABCA1 and TSLPR were detected by using RT-PCR and Western blotting. As compared with the control group, TSLP treatment significantly promoted lipid accumulation in macrophages, significantly increased protein expression of CD36 and TSLPR in a dose-dependent manner, and significantly reduced the expression of ABCA1 protein in a dose-dependent manner. No significant differences were noted between the TSLPR-antibody group and the control group. TSLP may down-regulate the expression of cholesterol efflux receptor ABCA1 and up-regulate scavenger receptor expression via the TSLPR signaling pathway, thereby promoting macrophage-derived foam cell formation.