Establishment of a Halophilic Bloom in a Sterile and Isolated Hypersaline Mesocosm

• Published in: Microorganisms (Basel) Vol. 11; no. 12; p. 2886
• Main Authors: Rhodes, Matthew E, Pace, Allyson D, Benjamin, Menny M, Ghent, Heather, Dawson, Katherine S
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.11.2023
• Subjects: aerobiome; Aerosols; Algae; Aquatic ecosystems; Bird migration; Chloride; Closed ecological systems; dispersal; Earth; Environmental aspects; Extreme environments; Great Salt Lake; haloarchaea; Haloarcula; Halophilic bacteria; Inoculum; Mesocosms; Microorganisms; Natural history; Osmosis; Osmotic pressure; Parking facilities; Physiological aspects; Potassium; Salinity; Salt; Stratosphere; Water circulation; Water column; United States; Great Salt Lake; United States > US; South Carolina; Dead Sea; dispersal; aerobiome; Great Salt Lake; haloarchaea; Haloarcula
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11122886

Extreme environments, including hypersaline pools, often serve as biogeographical islands. Putative colonizers would need to survive transport across potentially vast distances of inhospitable terrain. Hyperhalophiles, in particular, are often highly sensitive to osmotic pressure. Here, we assessed whether hyperhalophiles are capable of rapidly colonizing an isolated and sterile hypersaline pool and the order of succession of the ensuing colonizers. A sterile and isolated 1 m hypersaline mesocosm pool was constructed on a rooftop in Charleston, SC. Within months, numerous halophilic lineages successfully navigated the 20 m elevation and the greater than 1 km distance from the ocean shore, and a vibrant halophilic community was established. All told, in a nine-month period, greater than a dozen halophilic genera colonized the pool. The first to arrive were members of the Haloarchaeal genus . Like a weed, the rapidly colonized and dominated the mesocosm community but were later supplanted by other hyperhalophilic genera. As a possible source of long-distance inoculum, both aerosol and water column samples were obtained from the Great Salt Lake and its immediate vicinity. Members of the same genus, , were preferentially enriched in the aerosol sample relative to the water column samples. Therefore, it appears that a diverse array of hyperhalophiles are capable of surviving aeolian long-distance transport and that some lineages, in particular, have possibly adapted to that strategy.