LIN-23, an E3 Ubiquitin Ligase Component, Is Required for the Repression of CDC-25.2 Activity during Intestinal Development in Caenorhabditis elegans

• Published in: Molecules and cells Vol. 39; no. 11; pp. 834 - 840
• Main Authors: Son, Miseol, Kawasaki, Ichiro, Oh, Bong-Kyeong, Shim, Yhong-Hee
• Format: Journal Article
• Language: English
• Published: United States Korean Society for Molecular and Cellular Biology 30.11.2016; 한국분자세포생물학회
• Subjects: Animals; Caenorhabditis elegans - enzymology; Caenorhabditis elegans - growth & development; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; F-Box Proteins - genetics; F-Box Proteins - metabolism; HeLa Cells; Humans; Intestinal Mucosa - metabolism; Intestines - enzymology; Intestines - growth & development; Phosphoprotein Phosphatases - genetics; Phosphoprotein Phosphatases - metabolism; Transfection; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; 생물학; intestinal development; C. elegans; E3 ubiquitin ligase; LIN-23; CDC-25.2
• ISSN: 1016-8478; 0219-1032
• DOI: 10.14348/molcells.2016.0238

( ) utilizes two different cell-cycle modes, binucleations during the L1 larval stage and endoreduplications at four larval moltings, for its postembryonic intestinal development. Previous genetic studies indicated that CDC-25.2 is specifically required for binucleations at the L1 larval stage and is repressed before endoreduplications. Furthermore, LIN-23, the β-TrCP ortholog, appears to function as a repressor of CDC-25.2 to prevent excess intestinal divisions. We previously reported that intestinal hyperplasia in mutants was effectively suppressed by the RNAi depletion of . Nevertheless, LIN-23 targeting CDC-25.2 for ubiquitination as a component of E3 ubiquitin ligase has not yet been tested. In this study, LIN-23 is shown to be the major E3 ubiquitin ligase component, recognizing CDC-25.2 to repress their activities for proper transition of cell-cycle modes during the postembryonic intestinal development. In addition, for the first time that LIN-23 physically interacts with both CDC-25.1 and CDC-25.2 and facilitates ubiquitination for timely regulation of their activities during the intestinal development.